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PMLS MIDTERM > HEMATOLOGY > Flashcards

HEMATOLOGY Flashcards

1
Q

a scientific study of blood and its
component
Helps physicians diagnose
abnormalities in the blood

A

HEMATOLOGY

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2
Q

The standard procedure of blood
collection uses lancets and needles of
various gauges.

A

Phlebotomy

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3
Q

3 TYPES OF PHLEBOTOMY

A

Skin puncture, venipuncture, arterial puncture

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4
Q

Venipuncture is involved with what vessel?

A

veins

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4
Q

Skinpuncture is involved with what vessel?

A

capillaries

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5
Q

Arterial puncture is involved with what vessel?

A

Arteries

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6
Q

Clear yellow liquid, Water, sugar, fat, protein, and salt
solution, 55% of a normal human’s blood volume

A

Plasma

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7
Q

■ Transport’s RBC, WBC, and platelets
through the blood vessels
■ Remove waste products of metabolism

A

Plasma

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8
Q

ERYTHROCYTE is what cell?

A

RED BLOOD CELL

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9
Q

The anucleated cell produced from
the bone marrow; Hemoglobin

A

RED BLOOD CELL

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10
Q

gas transporting protein
molecule

A

Hemoglobin

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11
Q

What are the functions of RBC?

A

■ Transports oxygen (lungs →
tissue)
■ Transports carbon dioxide (back
to lungs)

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12
Q

low number of RBCs

A

Anemia

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13
Q

Polycythemia

A

high number of
RBCs

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14
Q

(LEUKOCYTE)

A

WHITE BLOOD CELL

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15
Q

○ Nucleated cells that lack hemoglobin
○ Acts as a defense against infection

A

WHITE BLOOD CELL

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16
Q

What are the different types of WBC?

A

Neutrophil, lymphocyte, eosinophil, basophil, monocyte

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17
Q

○ Most numerous
○ Multi-lobed nucleus
○ Pale lilac granules
○ Function: Immune defense

A

NEUTROPHIL

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18
Q

○ Spherical nucleus
○ “Robin’s egg blue” cytoplasm
Types: ■ T- Cell: Cellular immune response
■ B Cell: Antibody production
■ NK Cells: kills cancer cells

A

LYMPHOCYTE

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19
Q

Cellular immune response

A

T- Cell

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20
Q

Antibody production

A

B Cell

21
Q

kills cancer cells

A

NK Cells

22
Q

○ Usually with a bilobed nucleus
○ Granules stain bright reddish-
orange
○ Function:
■ Defense against parasites
■ Active allergic response

A

EOSINOPHIL

23
Q

○ With purple-blue granules
○ Function:
■ Inflammatory response
■ Involved in allergic response

A

BASOPHIL

24
Q

○ Kidney-shaped or horseshoe-
shaped nucleus
○ Types (in tissues):
■ Dendritic cell
■ Macrophage

A

MONOCYTE

25
Q

Marks out cells that are antigens (foreign
bodies) that should
be destroyed by
lymphocytes

A

Dendritic cell

26
Q

Acts as antigen-presenting cells

A

Macrophage

27
Q

○ Cell fragments
○ Function:
■ Form clots during injury to
prevent blood from leaking
out

A

PLATELETS (THROMBOCYTE)

28
Q

HEMATOLOGY SECTION, HEMOGLOBIN DETERMINATION, HEMATOCRIT DETERMINATION, BLOOD CELL COUNT, BECKMAN COULTER AUTOMATED
HEMATOLOGY ANALYZER, PERIPHERAL BLOOD SMEAR

A

TESTS PERFORMED IN HEMATOLOGY
SECTION

29
Q

RETICULOCYTE COUNT, ERYTHROCYTE SEDIMENTATION RATE, PLASMA COAGULATION ASSAYS,

A

ADDITIONAL HEMATOLOGY PROCEDURES

30
Q

○ Hemoglobin
○ Hematocrit
○ Red blood cell count
○ White blood cell count
○ Platelet count/estimate
○ Rbc indices (MCV, MCH, MCHC)

A

Complete Blood Count (CBC)

31
Q

○ Counting the number concentration of
cells
○ Determining the relative distribution of
various types of cells
○ Measuring biochemical abnormalities
of the blood
○ Hemostasis and coagulation assays

A

Procedures performed in Hema Section

32
Q

Iron-containing oxygen transport
metalloprotein in the red blood cells

A

HEMOGLOBIN

33
Q

■ Cyanmethemoglobin Method
■ Automated Hemoglobinometry
■ Point-of-care (POC) Hemoglobin

A

HEMOGLOBIN DETERMINATION

34
Q

○ Reagent: Drabkin’s Reagent
○ Principle: Oxidation of ferrous
iron to ferric by potassium
ferricyanide =

A

Cyanmethemoglobin Method

35
Q

Utilizes cyanmethemoglobin
method with modified Drabkin’s
reagent

A

Automated Hemoglobinometry

36
Q

○ HemoCue Method
○ Modified azide methemoglobin
reaction
○ Regent:
■ Sodium nitrate and
sodium azide

A

Point-of-care (POC) Hemoglobin
Assay

37
Q

For evaluation or treatment of anemia and
determine the presence of nutritional deficiencies

A

HEMATOCRIT DETERMINATION

38
Q

Manual puncture, Blood collection method: skin
puncture, Spin a blood-filled capillary tube
using a microhematocrit
centrifuge (Method of Hematocrit Determination)

A

Spun Microhematocrit

39
Q

Computed from the mean
cell volume and the red cell
count (Method of Hematocrit Determination)

A

Automated

40
Q

■ White blood cells count
diluting fluid
■ Thoma white pipette
■ Hemacytometer and coverslip
■ Alcohol pads
■ Microscope

A

Requirements of Blood Cell Count

41
Q

Also known as Coulter Principle; The sizing and counting of
particles are based on
changes in electrical
resistance creating voltage
pulses.

A

ELECTRICAL IMPEDANCE

42
Q

■ Hydrodynamic focusing

method

■ Uses laser light in cell
counting and sizing

A

OPTICAL DETECTION

43
Q

a quantitative and automated hematology
analyzer, which is designed for in vitro
diagnostic use in clinical laboratories

A

BECKMAN COULTER AUTOMATED
HEMATOLOGY ANALYZER

44
Q

Steps in Peripheral Blood Smear

A
  1. Place a small drop of whole blood on a very
    clean slide. Hold a second slide at the angle
    above.
  2. While maintaining contact with the button
    slide, pull the top slide back to contact the
    drop, which will spread by capillary action.
  3. Maintain firm contact with the button slide and
    push the top slide in one motion to produce
    the smear.
45
Q

Young RBCs without nucleus but still
bear cytoplasmic RNA

A

RETICULOCYTES

46
Q

● Determines how the bone marrow produce and
release new RBCs to compensate lost/damaged
RBCs
● Blood film is stained with a supravital stain

A

RETICULOCYTE COUNT

47
Q

The rate at which RBCs fall in a column; non-specific test for inflammation

A

ERYTHROCYTE SEDIMENTATION RATE

48
Q

Anticoagulant for Erythrocyte Sedimentation Rate

A

Sodium Citrate

49
Q

Time of testing for Erythrocyte Sedimentation Rate

A

one hour

50
Q

Prothrombin Time (PT) and Activated Partial
Thromboplastin Time (APTT)

A

PLASMA COAGULATION ASSAYS

51
Q

○ Coagulation testing
○ Detect abnormalities in hemostasis
○ Anticoagulant: Sodium citrate
○ Sample: Plasma only

A

Prothrombin Time (PT) and Activated Partial
Thromboplastin Time (APTT)

PMLS MIDTERM (3 decks)

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