Hematology 1

Question 1

What are three reasons for anemia?

Answer 1

1) Bone marrow not working
2) Blood loss (eg hemorrhage)
3) RBC’s bring destroyed. Eg, parasite or IMHA.

Question 2

Which species has nucleated RBC’s?

Answer 2

NON-mammals

Question 3

What is the MOST common reason for low lymphocytes? Esp which species?

Answer 3

Stress. Cats

Question 4

What is the #1 thing that causes Heinz body formation? Esp cats.

Answer 4

Acetaminophen.

Question 5

What is the stain for RBC’s? For reticulocytes?

Answer 5

RBC's= Wright stain
Reticulocytes= BRILLIANT CRESYL BLUE

Question 6

What should you think when you see HIGH albumin levels?

Answer 6

Dehydydration!

Question 7

ALT is mostly ___ specific.

Answer 7

Liver

Question 8

PU/PD can ___ electrolyte levels.

Answer 8

Decrease (b/c all getting flushed out)

Question 9

How do you calculate the Absolute nucleated blood cell count?

Answer 9

% x total number of nucleated cells

part/whole=%

Question 10

How do you calculate the Absolute reticulocyte count?

Answer 10

% reticulocytes x number of RBC’s

Question 11

What would happen if you contaminated the contents of the red top tube with EDTA or Citrate?

Answer 11

Your sample in the red tube would have abnormally low levels of Ca and high levels of K+. The Ca levels would be so low the animal “shouldn’t be alive”.

Question 12

Name all the tubes and what they contain.

Answer 12

red- nothing
purple- EDTA
green- Heparin
blue- Citrate
red and black- barrier to separate serum from RBC's, prevents utilization of Glc by RBC's.

Question 13

How much blood is needed for a CBC or biochemical profile?

Answer 13

5ml

Question 14

What is the smallest size needle you should use for filling tubes?

Answer 14

20G

Question 15

You have to fill both a red and a purple tube. Which should you fill first?

Answer 15

Purple, but be careful not to contamination red top tube!!

fill purple first b/c time is of essence and don’t want blood to clot

Question 16

What could happen if you don’t fill the purple tube up high enough?

Answer 16

Too much EDTA/RBC ratio> RBC’s shrink. Erroneously low PCV and MCV,

Question 17

How soon after drawing blood should you analyze it?

Answer 17

one hour (or make a blood film and refrigerate tube).

Question 18

What are the three components of the buffy coat?

Answer 18

1) Leukocytes
2) Nucleated erythrocytes
3) Platelets

Question 19

What is yellow plasma suggestive of?

Answer 19

Icterus

Question 20

1) ^ Tprot and ^ PCV= ___.

2) Decreased T Prot and Decreased PCV= ___.

Answer 20

• ^ Tprot and ^ PCV= DEHYDRATION

Decreased T Prot and Decreased PCV= Blood loss