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HEMATOLOGY > Hematological Determinations > Flashcards

Hematological Determinations Flashcards

1
Q

1 g of Hemoglobin is qual to how many mL of oxygen?

A

1 g Hb = 1.34 mL of Oxygen

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2
Q

Reagent use for Acid Hematin

A

0.1 Normal HCl

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3
Q

reagent use for alkali hematin

A

0.1 normal NaOH

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4
Q

enumerate the 3 direct visual colorimetric method

A

acid hematin, alkali hematin, & tallquist method

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5
Q

Considered the quickest method of assessing
a patient’s hemoglobin level

A

Tallquist method

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6
Q

Direct method done by dropping blood in a special type of paper and compare w/ the colored chart

A

tallquist method

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7
Q

most reliable and recommended method for
Hgb measurement

A

Indirect (Cyanmethemohlobin)

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8
Q

Cyanmethemoglobin is able to measure all forms of Hgb except

A

Sulfhemoglobin

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9
Q

Colorometric method that is not affected by coagulation

A

Cyanmethemoglobin

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10
Q

reagent use for cyanmethemoglobin method

A

Detergent-modified Drabkin’s reagent

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11
Q

purpose of detergent in cyanmethemoglobin method

A

enhance lysis of RBC; decreased turbidity

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12
Q

component of drabkin’s reagent that Converts hemoglobin to hemiglobin

A

potassium ferricyanide

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13
Q

components of drabkin;s reagent that

A

Adds the cyanide ions to producehemiglobin cyanide(HiCN) w/ci s measured at a spectra of 540nm

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14
Q

Shortens the time needed for
complete conversion of Hb to HiCN

A

Dihydrogen potassium phosphate

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15
Q

Considered the one w/ greatest accuracy because it estimates oxygen combining power of the blood

A

Gasometric (Van Slyke Capacity Method)

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16
Q

Impractical because of using big instrument

A

Gasometric

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17
Q

DRAWBACK: Affected by myeloma proteins, abnormal globulins, radiographic contrast
media

A

Copper Sulfate Method

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18
Q

Use to screen blood donors before

A

Copper Sulfate Method

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19
Q

SG of copper sulfate =

A

1.053

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20
Q

SG of copper sulfate = to how much of Hemoglobin?

A

SG of copper sulfate = 12.3 g/dL of Hgb

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21
Q

1 g of Hb is equal to how many content of iron?

A

3.47 mg but 3.35 if according to Alba

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22
Q

Measurement of carboxyhemoglobin
concentration in the blood

A

Katayama’s test

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23
Q

reagent use in katayama’s test

A

ammonium sulfide

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24
Q

Positive COHgb =

A

rose red color

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25
Q

PACKED CELL VOLUME (PCV); Measures the concentration of RBCs in a given volume of whole blood in a capillary
tube

A

Hematocrit

26
Q

clarifying various degrees of anemia & monitoring acute hemorrhagic conditions

A

Hematocrit

27
Q

hematocrit is one of the basis for diagnosing ——

A

polycythemia

28
Q

Macromethod that used sodium oxalate as the main reagent

A

Van Allen
Sanford-Margath
Haden

Mnemonic: VaSaHa

29
Q

reagent or main component use for bray macromethod

A

Heparin

30
Q

Speed of Centrifugation of macromethod

A

2000-2300 g (30 mins)

31
Q

tube Use for Hct & ESR

A

Wintrobe Tube

32
Q

length of wintrobe tube

A

11.5 cm

33
Q

bore size of wintrobe tube

A

3 mm

34
Q

Wintrobe tube’s Calibration mark (ESR):

A

0-100 (white mark)

35
Q

Wintrobe tube’s Calibration mark (Hct)

A

10-0 (red mark)

36
Q

Most common method because of using less blood; less time consume; better packing ofcells

A

Micromethod (Adam’s method)

37
Q

speed of centrifugation of micromethod

A

10,000-15,000 g (5 mins)

38
Q

Microhematocrit tube length

A

70-75 mm or 7 cm

39
Q

microhematocrit tube bore size

A

1 mm

40
Q

Length of Sealing Clay:

A

4-6 mm

41
Q

Source of errors: overcentrifugation

A

falsely decrease

42
Q

Source of errors: inadequate centrifugation

A

falsely increase

43
Q

Source of errors: Improper sealing

A

falsely decrease

44
Q

Source of errors: Short draw

A

Falsely decrease

45
Q

Source of errors: Introduction of interstitial fluid from a skin puncture

A

falsely decrease

46
Q

best diluting fluid for RBC count

A

Dacie’s

47
Q

Dilution factor of RBC count

A

200

48
Q

Hgb is how many grams higher in male?

A

1-2 g/dL

49
Q

Components of Turk’s diluting fluid

A

Gentian violet & Glacial acetic acid & hydrogen peroxide

50
Q

characteristic of WBC diluting fluid

A

hypotonic

51
Q

Dilution ratio of WBC

A

20

52
Q

Phase contrast microscopy method
 Uses 1 % ammoniumoxalate ADVANTAGE: increase refractility; considered as a reference method for platelet count

A

Brecker-Cronkite Method

53
Q

3 components of Rees-Ecker diluting fluid

A

sodium citrate, brilliant cresyl blue, and formalin

54
Q

Fragments of leukocyte cytoplasm that are sometimes
numerous in leukemias may falsely _____ the count

A

elevate; increase

55
Q

These can occur if platelets adhere to neutrophils
agglutinins, spontaneous aggregation, or incipient clotting due to faulty blood collection.

A

Plalelet satellism

56
Q

10 squares arranged in 2 horizontal rows with 5 columns each; each square is further subdivided into 16 smaller squares

A

Speir’s Levy

57
Q

A red cell measurement that quantitates cellular volume heterogeneity reflecting the
range of red cell sizes w/in a sample
 Useful in early classification of anemia

A

Red Cell Distribution Width (RDW)

58
Q

Applies only to samples that have a normocytic normochromic red blood cells

A

Rule of Three

59
Q

For Bone Maroow aspirates crush prep

A

Cover Glass Smear

60
Q

produces a uniform blood film, in which all cells are separated (a monolayer) and randomly distributed

A

Spinner Method

HEMATOLOGY (2 decks)

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