HEMA MIDTERMS COMPUTATIONS Flashcards
RBC Thoma Pipette Dilution
101-1/total amount of blood aspirated
RBC Count (1:200)
no. of RBC x 10,000
other RBC count formula
- Ave. cell # x dilution factor / area counted x chamber depth
- # of RBC x Area C.F x Depth C.F x Dilution C.F in 1/5 sq.mm
WBC count (1:20)
Total # of cells in all 4 squares x 50
OTHER formula for WBC
WBCs Counted x 10 x 20 / 4
Dameshek’s Method
Platelets x RBC Count / 1,000
Guy and Leake
Platelets per uL = Platelet x 5 x 10 x 100
Rees and Ecker Method (platelet count)
No. of cells x Dilution Factor x Depth Factor / Area Factor
Rees and Ecker Platelets/µL
Platelets Counted x 10 x 200 / 4
Brecher-Cronkite Method
Platelets per uL = Platelet x 5 x 10 x 100
Thrombocyte No. concentration
Platelet count x .01 = ______ x 10^9/L
WBC Differential Count & WBC Absolute Count
Neutrophil
47-49
1.8 - 7.7 x 10^9/L
WBC Differential Count & WBC Absolute Count
Lymphocyte
14-47
1 - 4.8 x 10^9/L
WBC Differential Count & WBC Absolute Count
Monocytes
2-11
0 - .8 x 10^9/L
WBC Differential Count & WBC Absolute Count
Eosinophils
0-7
0-.45 x 10^9/L
WBC Differential Count & WBC Absolute Count
Basophil
0-2
0-.30 x 10^9/L
WBC Differential Count & WBC Absolute Count
Stab or Band cell
0-7
Hgb Concentration in Chemical Method
Iron content (mg/dL) / 3.47
Cyanmethemoglobin
C test / C standard= A test/ A standard or C test = C standard x A test / A standard
Wintrobe Hematocrit (QNS)
L1 x 100 / L2
L1 - height of red cell column
L2 - height of entire column
Wintrobe Hematocrit (<5mL)
(Vol. of pRBCs/ Vol. of whole blood used) x 100
Haden’s Modification
Hct % = vol of pRBCs x 20
Haden’s Modification (<5mL)
Hct % = (vol of pRBCs / vol of whole blood used) x 100
MCV
MCV = Hct/RBC Countx 10
*
MCH
MCH = Hgb/RBC Count x 10
MCH
MCHC
MCHC = Hgb/Hct x 100
Rule of Three
Hemoglobin x 3 = Hematocrit ± 3
RBC x 3 = Hemoglobin ± 3
%Reticulocyte
No. of reticulocytes x 100 / 1000
Newborn (within 2 weeks): 2-6%
Adults: 0.5-1.5%
Absolute Reticulocyte Count
(Reticulocytes (%)/ 100) x RBC Count x 1000
RBC Count is in (x1012/L) format
Retics/mm3
(% retics / 100) x RBC count in millions
Reticulocyte No. Concentration
Retics/mmm3 x .001 = ___ x 10^9/L
Corrected Reticulocyte Count
[Reticulocytes % x Hct (%)] / Normal Hct
Retics/uL
Reticulocytes (%) / 100 x RBC Count
Reticulocyte Production Index
CRC / Maturation days of Retics in blood
Zeta Sedimentation Rate (ZSR)
ZSR% = (HCT (%) / Zetacrit) x 100