HEMA LAB EXAMS Flashcards

1
Q

What is the greak word of blood?

A

Haima

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2
Q

What is the greek word of study or science

A

Logos

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3
Q

What provides for the analysis of normal and pathogenic peripheral blood cells, hematopoetic tissue, and the cells in non-vascular body cavities such as CSF and serous fluid?

A

Clinical laboratory hematology

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4
Q

Who describe worms in the blood in 1657?

A

Anthanasius Kircher

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5
Q

Who discovered erythocytes? What year?

A

Swammerdam, in 1658

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6
Q

Anton Van Leeuwenhoek described what in 1674?

A

Human erythrocytes

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7
Q

What year did platelets were described?

A

1842

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8
Q

Who distinguished PMN from other leukocytes in 1846?

A

Wharton Jones

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9
Q

First complete classification of leukocytes by.. ? In .. ?

A

Ehrlich, 1879

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10
Q

Development of ___ stain in 1902 by __?

A

Wrights stain, by James Homer Wright

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11
Q

In what year was Hematology considered as a branch of science from clinical medicine and pathology?

A

1920

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12
Q

A life sustaining unit. Carries oxygen and nutrients to the tissues and waste products to the lungs, liver, and kidney.

A

Blood

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13
Q

55% of the total blood. It is the liquid portion of unclotted blood?

A

Plasma

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14
Q

Consists of platelets and leukocytes, <1% of the total blood.

A

Buffy coat

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15
Q

45% of the total blood

A

Erythrocytes

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16
Q

The fluid that remains after coagulation?

A

Serum

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17
Q

Blood is in fluid?

A

In vivo

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18
Q

It coagulates in 5-10 mins

A

Blood, In vitro

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19
Q

Thick and viscous, ___ times thicker than water

A

3.5 - 4.5 times

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20
Q

Makes up ___ % of the total body component or 60 - 80ml/kg

A

6-8%

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21
Q

Blood is approximately __ gms solid per 100mL

A

20 gms

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22
Q

Total blood volume (TBV) for an adult male?

A

5-6L

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23
Q

Total blood volume (TBV) for an adult female?

A

4-5L

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24
Q

Total blood volume (TBV) for a newborn?

A

250 - 350mL

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25
Q

Normal pH of blood?

A

7.35 - 7.45 (ave of 7.40)

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26
Q

pH of:
Venous blood __
Arterial blood __

A

7.35
7.45

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27
Q

Specific gravity of whole blood?

A

1.045 - 1.066

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28
Q

SG of serum?

A

1.024 - 1.028

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29
Q

SG of plasma?

A

1.025 - 1.029

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30
Q

Ratio of the density of a substance to the density of a reference substance

A

Blood specific gravity or specific gravity

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31
Q

Measures acidity and basicity of an aqueous solution

A

pH

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32
Q

Color of:
Arterial blood ___
Venous blood ___

A

Bright red
Dark purplish red

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33
Q

Pulmonary arteries and veins color
W/ O2:
W/o O2:

A

Purple
Blue

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34
Q

Device used for measurement of osmolality

A

Osmometer

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35
Q

Concentration of solutes dissolved in the blood

A

Osmolality

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36
Q

Reference range for osmolality

A

281 - 297 milli-osmoles per kg

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37
Q

Give atleast 3 function of blood

A

Respiratory, nutritional, excretory, buffering action, maintenance of constant body temp, transportation of hormones and other endocrine secretion, body defense mechanism

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38
Q

What does OSHA stand for?

A

Occupational Safety and Health Administration

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39
Q

When did OSHA took effect?

A

March 6, 1992

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40
Q

What are the bloodborne pathogens?

A

HIV, HBV, HCV, Syphilis, Malaria

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41
Q

How long does HBV lasts?

A

1 week

42
Q

Physiologic factor that affects test results:
Increases protein, Iron, Cholesterol

A

Posture

43
Q

Physiologic factor that affects test results:
- Cortisol, TSH, Fe
- Eos

A

Diurnal rhythm

44
Q

Physiologic factor that affects test results:

Increseas creatine, total protein, creatine kinase, Myoglobin, Aspertate Aminotransferase, WBC, HDL

A

Exercise

45
Q

Physiologic factor that affects test results:

Anxiety and excessive crying in children

A

Stress

46
Q

Physiologic factor that affects test results:

Increases glucose and lipid

A

Fasting <2hrs (Diet)

47
Q

Physiologic factor that affects test results:

Increase in WBC, cortisol

A

Smoking

48
Q

You will conduct an FBS test on a px, but the px said he smoked a while ago. Would you accept or deny?

A

Deny, smoking increases cortisol or blood glucose thus resulting to a false increase in Hgb

49
Q

Where will you apply the torniquet?

A

3-4 inches above the venipuncture site

50
Q

Recommended tubes by OSHA?

A

Plastice collection tubes

51
Q

Sterile, available in a variety of lengths and gauges

A

Needle

52
Q

Give the size and color of the needle used for blood donation

A

16 guage, Gray color

53
Q

Give the size and color of the needle used for newborn or in pedia

A

23 guage, orange color

54
Q

Give the size and color of the commonly used needle for phlebo (in adults)

A

21 guage, purple color

55
Q

Designed to comply with OSHA’s revised Occupational Exposure to Bloodborne Pathogens

A

Needle holder

56
Q

Consists of a short needle with plastic wings connected to thin tubing

A

Winged Blood Collection Set or Butterly needle

57
Q

Consists of a barrel, graduated in milliliters, and a plunger

A

Syringe

58
Q

Gove the parts of a syringe

A

Plunger, barrel, hub, needle

59
Q

Most common antisepsis __

A

70% alcohol

60
Q

Clean the venipuncture site in a ___?

A

Circular motion, beginning in the center working outward

61
Q

Give the selection of vein from 1st to last priority

A

1st median vein
2nd cephalic vein
3rd basilic vein

62
Q

Degree angel of the syringe for venipuncture

A

30°

63
Q

Order of draw for veni puncture

A
  1. Blood culture - yellow
  2. Coagulation tube - light blue
  3. Serum tube - red, gold, orange, red-gray
  4. Heparin tube - green or light green
  5. EDTA tube - lavander or pink
  6. Sodium flouride w/ or w/o EDTA - gray
64
Q

What does EDTA Sstand for?

A

Ethylenediaminetetraacetic acid

65
Q

Ratio of blood to anticoagulant

A

9:1

66
Q

Complications encountered in veni

A

Ecchymosis, hematoma, petechiae

67
Q

Rupture of blood cells with the consequent escape of Hgb

A

Hemolysis

68
Q

Swelling caused by abnormal accumulation of fluid

A

Edema

69
Q

Px has the right to refuse to give blood spx

A

Patient refusal

70
Q

Collection site for <1 year old

A

Lateral, medial plantar surface of the heel

71
Q

What are the normal adult counts
Males:
Females:

A

Males: 4.5 - 5.9 x 10^12/L
Females: 4.0 - 5.2 x 10^12/L

72
Q

A condition characterized by abnormally low levels of healthy RBC or Hgb

A

Anemia

73
Q

Increase in the no. of circulating erythrocytes of more than two stand. devi. above the mean normal

A

Erythocytosis

74
Q

Increase in the no. of RBC

A

Polycythemia

75
Q

Acute or chronic neoplastic diseases of the bone marrow in which unrestrained proliferation of WBC occurs

A

Leukemia

76
Q

All cell types can be counted this way

A

Manual cell count

77
Q

Steps involved in Manual cell counts
1. Dilution
2. ___
3. ___
4. Computation

A
  1. Charging
  2. Counting
78
Q

Manually adding accurate amounts of blood and dilution fluid in a test tube

A

Manual dilution

79
Q

Diluting fluid for WBC

A

1% hydrochloric acid or 3% acetic acid or 1% ammonium oxalate

80
Q

Diluting fluid for RBC

A

Isotonic saline

81
Q

Diluting fluid for platelets

A

1% ammonium oxalate

82
Q

Most common chamber?

A

Levy chamber

83
Q

Rules for counting

A

Cells touching the Top and left boundaries are counted (1st and mid line)
Cells touching the right and bottom boundaries are not included ( _l )

84
Q

Instrument used to count the blood cells

A

Hemocytometer

85
Q

Different types of hemocytometer

A

Neubauer
Fuch’s Rosenthal
Levy’s chamber

86
Q

Difference between RBC and WBC pipette

A

Rbc: red bead, upto 101 mark, bulb is larger, lumen is smaller
Wbc: white bead, upto 11 mark, bulb is smaller, lumen is larger

87
Q

Dilution factor for RBC counting is ___

A

200

88
Q

Dilution factor for WBC is __

A

20

89
Q

Magnification for
WBC : __ x
RBC : __ x

A

10x LPO
40x HPO

90
Q

Distance between counting chamber and the cover slip?

A

0.1 mm

91
Q

Total area of RBC squares

A

0.2 mm^2

92
Q

Total area for WBC squares

A

4 mm^2

93
Q

Sinong manlilibre ng ice cream, at isang meal kay King?

A

Mundi, at Celeste

94
Q

Drabkin’s reagent composition

A

Potassium cyanide, potassium ferricyanide, potassium dihydrogen phosphate

95
Q

Inhibits the metabolism of glucose by blood cells

A

Antiglycolytic agent

96
Q

Prevent blood from clotting

A

Anticoagulant

97
Q

How many inversions for gold/red-gray tubes?

A

5 inversions

98
Q

How many inversions for red tubes,
glass
plastic

A

0 - Glass
5 - plastic (with clot activator)

99
Q

How many inversions for light blue

A

3 - 4 inversions

100
Q

How many inversions for EDTA tubes (lavander)

A

8 inversions

101
Q

How many inversions for green tubes

A

8 inversions

102
Q

Proper way of tube inversions?

A

Figure of eight motion (?)