Hema 2

Question 1

Tonkantin method other name

Answer 1

Rees and ecker method

Question 2

Tonkantin rgts

Answer 2

Na citrate - ag
Formalin - preservative
Bcb - stain

Question 3

Brecker conkrite rgt

Answer 3

1 pct nh4 oxalate

Ref method

Question 4

Unopette method rgt

Answer 4

Edta and nh4 oxalate

Question 5

Unopette principle

Answer 5

Edta and nh4 oxalate chelates magnesium and calcium

Hemolysis of red cells by hypotonicity

Question 6

Plt esti

Plt count per oio

Answer 6

8-10 plts

Question 7

Plt estimate factor

Answer 7

20,000

Question 8

Reporting of plt estimate

Answer 8

0-49k marked decrease
50-99k moderately decreased
100-149k slightly decreased
150-199k low normal
200-400k normal
401-599k slightly increased 
600-800k moderately increased 
More than 800k markedly increased

Question 9

Significant plt levels

Answer 9

Less 100k abnormally low, ab bt
30-50k bleeding possible with trauma
Less 30k spon bleeding
Less 5 k sever spon bleeding

Question 10

Platelet aggregation test principle

Answer 10

Aggregating agents, ristocetin, adp, collagen, epi causes shape changein plts. Increase light transmittance is to increase aggregation

Question 11

Glass bead retention test

Answer 11

Salzaman test

Question 12

Glass bead reten test nv

Answer 12

26-60 pct

Question 13

Gbr test principle

Answer 13

When passing thru glass, normal plt would adhere

Formula= (pc1-pc2/ pc1) x 100

Question 14

Ehler danlos

Answer 14

Hereditary
Prolonged bt
Pos tourni test

Question 15

Pseudoxanthoma elasticum

Answer 15

Calcified connective tissues

Question 16

Hereditary telangiectasia

Answer 16

Thin and fragile veins. Abn dilated

Rendu osler weber syndrome

Question 17

Kasabach merrit syndrome

Answer 17

Hemangiomata

Tumor of the blood vessel

Question 18

Aplastic anemia

Answer 18

Generalized bonemarrow suppression leading to decrease in all cell types

Question 19

Myelopthisic process in quanti plt disorder

Answer 19

Space occupying lesions like leukemia, tumor, fibrosis

Question 20

Selective suppression of megakryocyte

Answer 20

Chrolothiazide

Question 21

Thrombocytopenia with absent radius

Answer 21

Absent or decreased bone marrow megakaryocytes

Question 22

Duckherts test rgt substi

Answer 22

1 pct monochloroacetic acid

2pct acetic acid

Question 23

Whole blood clot lysis

Answer 23

Stable clot for atleast 48 hours. Less is excessive fibrinolysis

Question 24

Euglobulin test

Answer 24

Pptd proteins using water and acid.
It prevents inhibition againts anti plasmins
It is rapid and sensitive

Lysis in less than 2 hours is excessive fibrino
Ppp+ acid
Euglo + thrombin = clot formation

Question 25

Protamine sulfate gelation test

Answer 25

Test for fibrinogen degradation products Paracoagulation - rplaces the secondary degra prod from fibrin monomers and primary fdps rx to gel formation Normally, no clot

Question 26

Ethanol gelation test

Answer 26

Less sensi but mor specific than prota in detecting mono and polymers in plasma 50 pct etha causes monomer dissociation, then polymerization, forming gel Normal, no gel formation

Question 27

Latex d dimer test

Answer 27

Test for d dimers Less than 4 hours after onset of dic already detectable

Question 28

X and y degrad prod

Answer 28

Ag

Question 29

Y and d degra

Answer 29

Inhib fibrin polymerisation

Question 30

E degrad

Answer 30

Powerful thrombin inhibitor