Heamatology Flashcards

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1
Q

How can the RBC and Hb of a newborn high?

A

This happens when cord clamping is delayed and blood from the placenta and umbilical artery re-enters the infant’s circulation.

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2
Q

How can haemoglobin be measured?

A

Hb can be estimated by a measurement of its colour, by determination of its power to combine with oxygen or carbon monoxide or by analysis of its iron content.

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3
Q

Why is there a decrease in Hb, Hct and RBC during normal pregnancy?

A

During normal pregnancy, there is an increase in erythropoietic activity and an increase in plasma volume, causing the red cell indices to decrease.

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4
Q

What are the factors that contribute to lower Hb in the elderly?

A

Renal insufficiency, inflammation, testosterone deficiency, diminished erythropoiesis, stem cell proliferative decline and myelodysplasia.

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4
Q

What are the factors that contribute to lower Hb in the elderly?

A

Renal insufficiency, inflammation, testosterone deficiency, diminished erythropoiesis, stem cell proliferative decline and myelodysplasia.

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5
Q

What are the factors that contribute to lower Hb in the elderly?

A

Renal insufficiency, inflammation, testosterone deficiency, diminished erythropoiesis, stem cell proliferative decline and myelodysplasia.

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6
Q

What is the recommended method to determine haemoglobin concentration in blood?

A

Haemiglobincyanide method

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7
Q

How to calculate haemoglobin concentration?

A

Hb (g/L) = (absorbance test sample/absorbance of standard) * concentration of standard * (dilution factor/1000)

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7
Q

How to calculate haemoglobin concentration?

A

Hb (g/L) = (absorbance test sample/absorbance of standard) * concentration of standard * (dilution factor/1000)

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7
Q

How to calculate haemoglobin concentration?

A

Hb (g/L) = (absorbance test sample/absorbance of standard) * concentration of standard * (dilution factor/1000)

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8
Q

What are the main advantages of using HbCN method to determine haemoglobin concentration?

A

It allows direct comparison with reference standards. All forms of haemoglobin except SHb are readily converted to HiCN.

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9
Q

What are the stains that react with reticulocyte?

A

Azure B, Brilliant cresyl blue and new methylene blue

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10
Q

How can you differentiate between the most immature reticulocyte and the least immature ones?

A

The most immature reticulocytes have the largest amount of precipitation material as compared to the least immature ones.

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11
Q

How can you differentiate between the most immature reticulocyte and the least immature ones?

A

The most immature reticulocytes have the largest amount of precipitation material as compared to the least immature ones.

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12
Q

How many groups are reticulocytes divided?

A

Four groups ranging from group I (most immature) to group IV (most mature)

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13
Q

How do you calculate MCV?

A

MCV = HCT/RBC

14
Q

How is MCH calculated?

A

MCH = Hb/RBC

15
Q

How is MCHC calculated?

A
MCHC = (MCH/MCV) x 1000
MCHC = (Hb x 1000) ÷ (MCV x RBC)
16
Q

Give examples of immature granulocytes?

A

Promyelocytes
Myelocytes
Metamyelocytes