Health and Safety

Question 1

What is the One-Glove Rule?

Answer 1

Removing one glove to open doors when going between labs and touching high-use areas like door handles, prevents contamination

Question 2

PPE required to mop up spills

Answer 2

Eye protection (glasses), gloves, lab coat

Question 3

Two disinfectants used to eliminate all microorganisms in the lab

Answer 3

Chloros and Tristel

Question 4

How to mop up small spills

Answer 4

PPE, cotton wool to absorb spill, contaminated waste put into grey autoclave bins

Question 5

How to mop up larger spills

Answer 5

PPE, precept granules poured and mixed into spill, take care not to inhale chlorine fumes, increase ventilation by opening windows

Question 6

What microorganisms is Tristel effective against?

Answer 6

Mycobacterium spp, fungi, spores, viruses

Question 7

What disinfectant is used to clean centrifuges?

Answer 7

Industrial methylated spirit (70% alcohol)

Question 8

When are the Cat 3 labs fumigated (with formaldehyde)?

Answer 8

Before servicing or after large spill in cabinets

Question 9

Are sharps bins fully closed before going into autoclave? + why?

Answer 9

No, so that clave steam can penetrate into bin
(then bin is shut fully and sent for incineration)

Question 10

What temperature and time does the autoclave usually go for? + range of other cycles

Answer 10

127°C for 1 hour, 121°C to 134°C

Question 11

What temperature is required for incineration?

Answer 11

1200°C

Question 12

What’s the estates extension number?

Answer 12

20600 (reporting leaking taps, toilet cisterns, etc)

Question 13

What does Chloros kill?

Answer 13

Bacteria, fungi, flu virus, etc