HCT lab module 2 exercise 2

Question 1

Branch of histology that deals with the process of examining tissues in order to know more about the disease and its root cause.

Answer 1

Histopathology

Question 2

Process wherein pathologists are able to reach diagnosis by examining a small piece of tissue from various organs.

Answer 2

Biopsy

Question 3

Professional that study the tissues processed by histotechnicians/histotechnologists to come up with a diagnosis based on the history of the patient.

hint: type of pathologist

Answer 3

Histopathologist

Question 4

[T or F] Tissue samples commonly arrive in the laboratory before surgical operation.

Answer 4

False - tissue sample arrive following/after surgical operation

Question 5

This is where tissue samples are placed for preservation and hardening, and it’s subsequent sectioning.

Answer 5

fixative reagent

Question 6

The end product of tissue preparation

Answer 6

Tissue block

Question 7

At what temperature is a paraffin oven set at to melt the wax used in embedding tissue samples.

Answer 7

55-60 degrees celsius

Question 8

What laboratory equipment is used to melt the wax used for embedding tissue samples

Answer 8

paraffin oven

Question 9

• suitable for holding and identifying tissue sample blocks

- fits well in microtome chuck adapters

Answer 9

Embedding rings

Question 10

This is where an identification number is marked before being place on top of a tissue block in tissue preparation.

Answer 10

Etched writing surface on the Embedding ring

Question 11

Two levels of processing specimens for examination

Answer 11

1. Tissue preparation

2. Slide preparation

Question 12

Used to cut very thin sections of tissue sample

Answer 12

Microtome

Question 13

Used to float paraffin ribbon, to stretch sections and remove wrinkles and folds that might interfere in microscopy

Answer 13

Floatation/ Water bath

Question 14

Why are floatation/ water bath used during mounting?

Answer 14

To preserve delicate tissue slides

Question 15

• allows tissue to relax and smooth out prior to being mounted to tissue slide
• also makes paraffin stick to the slide

Answer 15

warm water

Question 16

water bath is heated at what temperature which is below the melting point of paraffin.

Answer 16

5 to 10 degrees celsius

Question 17

Applied on the slide before sections are scooped out of the water so that tissue will remain attached to the slide during staining

Answer 17

Adhesives

Question 18

What is the temperature for floatation/water bath in most laboratories?

Answer 18

45-50 degrees celsius

Question 19

Melting point of common waxes

Answer 19

45, 52, 56, and 58 degrees celsius

Question 20

Equipment with vertically grooved interior wall used for staining

Answer 20

Coplin jars

Question 21

Staining equipment that can hold maximum of 6 slides? 10 to 20 slides?

Answer 21

Coplin jars; Staining dishes

Question 22

This prevents evaporation and contamination of reagents

Answer 22

Lids/Caps

Question 23

Done in order to protect tissue slides from damage and allow storage for future reference in slide box

Answer 23

Tissue slides are overlaid with mountant and cover slips

Question 24

Tissue preparation 4 general steps:

Answer 24

1. Tissue samples received from surgical operation
2. Tissue samples placed in fixative agent
3. Tissue samples are placed in cassettes to be cut in thin pieces
4. Tissue sample is embedded in wax to create tissue block

Question 25

Slide preparation 5 general steps:

Answer 25

1. Tissue block is cut into thin sections by microtome
2. Tissue section is mounted on a slide
3. Excess paraffin is cut out by stretching table
4. Tissue staining
5. Tissue specimen is overlaid with mountant and cover slip