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HCLD 101 > HCLD 101 > Flashcards

HCLD 101 Flashcards

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1
Q

what % atmospheric oxygen should trigger lab alarm

A

18%

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2
Q

What is the oxygen level at which a worker can asphyxiate and die

A

10%

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3
Q

UN3373

A

category B label for non hazardous biological specimens

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4
Q

UN2814

A

infectious substances only to humans

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5
Q

UN2900

A

infectious only to animals

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6
Q

how should ethanol be labelled

A

flammable

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7
Q

what is a Cusum plot

A

cumulative sum chart to detect deviation from the mean

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8
Q

what are Westgard rules

A

statistical rules to determine under what circumstances a run should be rejected

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9
Q

what is a levy-jennings plot

A

plotting results against the mean. 2sd either side

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10
Q

medicare is for ?

A

old people

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11
Q

medicaid is for?

A

poor people

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12
Q

CMS

A

center for medicare and medicaid services

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13
Q

what is stark law

A

must not refer to lab that owns another lab/who has interests

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14
Q

age discrimination in employment act

A

ADEA 1967

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15
Q

the perported benefits of co-culture are all of the following except: a: prod of ammonia that stims embryo devel bprod of embryotoxic factors c: rel growth factors into CM c; removal of toxins from medium

A

A

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16
Q

which of the following may be added to proteins in human serum: q vit/gluc and fatty acids b: gluc, hormones and vit c; fatty acids, glu and hormaones d: hormones, vit and fatty acids

A

D

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17
Q

glutathione is a marker of what in the oocyte

A

cytoplasmic maturation

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18
Q

sperm gain potential to fertilize at which stage

A

during passage through female tract

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19
Q

what are the sequences of events in the egg after fert:

A

intracellular Ca release-meiotic reactivation-separation of chromatids

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20
Q

preimplantation cleaveg is charcterised by what frequenct

A

initially slow follwed by more rapid cleavage

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21
Q

implantation is categorized by what events

A

hatching-apposition-invasion-syncytialization

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22
Q

2 main functions of amniotic fluid are:

A

shock absorber and buffer temp changes

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23
Q

primary follicle devl involves which changes:

A

granulosa cell prolif and increase in oocyte vol

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24
Q

LH surge is triggered under what hormonal conditions

A

high E2 decreasing FSH

25
Q

how many eggs left at puberty

A

400,000

26
Q

how many eggs at birth

A

2,000,000

27
Q

how many eggs at menopause

A

0

28
Q

primordial follicle is unstimulated

A

primary follicle has started stim

29
Q

In an adult human female, the development of a primordial follicle containing an oocyte to a preovulatory follicle takes how long?

A

in excess of 120 days.

30
Q

primary oocyte lives in the primordial follicle, is it haploid or diploid

A

diploid

31
Q

dictyate or dictyotene

A

prolonged resting of primary oocyte phase up to 50 years

32
Q

what do connexin proteins do in oocyte?

A

gap juction proteins maintain cooyte arrest

33
Q

what is the muscosal layer of the vag mainly comprised of

A

stratified squamous eplithelium and no secretory cells

34
Q

endometrium is in 3 layers. 2 are sensitive to steroid stim and are called stratum functionalis. what are the 2 layers called:

A

stratum spongiosum and stratum compactum

35
Q

how many eggs by 5th month of gestation

A

6-7 million

36
Q

ratio of ICM to TE cells:

A

1:3

37
Q

primordial follicles-primary follicles-secondary follicles what is egg stage in each

A

primary oocyte-secondary oocyte only at ovulation

38
Q

is a morula charachterised by a polarized outer cell population?

A

yes

39
Q

when does developmental failure occur most often?

A

3rd meiotic division and blastulation

40
Q

best indictaor of embryo competency

A

ICM

41
Q

aneuploidy

A

multinucleation

42
Q

what is a centimorgan

A

distance between chromosome positions for which there is 1% chance of a crossover

43
Q

are mitochondrial disorders typically mosaic for the condition

A

YES

44
Q

does the presence of h2O2 prod lactobacillus enhance implantation?

A

YES

45
Q

Does vit cryoprotectant have high or low MW

A

HIGH

46
Q

what is cell lysis caused by during thawing

A

osmotic stress

47
Q

FSH

A

secreted by ant pit in response to GnRH from hypothalamus.

48
Q

E2

A

secreted by granulosa cells which use androgens from theca cells to make E2

49
Q

action of E2

A

grows follicles and stimulates endo thickening

50
Q

P4

A

prod by CL formed after ovulation and then by placenta

51
Q

hCG

A

prod by trophectoderm

52
Q

LH

A

prod by ant pit. Surge initiates ovulation and occurs in response to high E2 levels

53
Q

FSH receptors on what cells

A

granulosa and sertoli cells

54
Q

sertoli cells do what?

A

make inhibin (negative feedback) and androgen binding protein

55
Q

leydig cells produce what?

A

testosterone in response to LH

56
Q

hormonal control of spermatogenesis

A
57
Q

semen cut off variables for all WHO editions

A
58
Q

In the Dr. Menkveld presentation, no WHO 5th vs. WHO 4th Morphology criteria differences are mentioned, only a lower reference limit. Strict criteria is used for morphology assessment and grading in both manuals.

  • The concept of normal spermatozoon by WHO 4th and 5th manuals are the same.
  • Most of WHO 4th and 5th Morphology criteria are the same.
  • There are the slight but not significant differences in some dimensional details that were updated in the WHO 5th manual.

• The WHO 4th manual Morphology reference value was not final and was updated in the WHO 5th edition.
The overall conclusion is that the WHO 4th and 5th Morphology criteria are similar, as they are based on the same strict principles, similar guidelines and reference publications. As the WHO 4th and 5th criteria are similar, we did not conduct any additional study for the WHO 5th morphology algorithm and stayed with the same developed for the WHO 4th. Only the reference value was changed in the V-Sperm.

A

difference between WHO 4th and 5th

59
Q
A

HCLD 101 (1 decks)

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