HCLD 101 Flashcards
what % atmospheric oxygen should trigger lab alarm
18%
What is the oxygen level at which a worker can asphyxiate and die
10%
UN3373
category B label for non hazardous biological specimens
UN2814
infectious substances only to humans
UN2900
infectious only to animals
how should ethanol be labelled
flammable
what is a Cusum plot
cumulative sum chart to detect deviation from the mean
what are Westgard rules
statistical rules to determine under what circumstances a run should be rejected
what is a levy-jennings plot
plotting results against the mean. 2sd either side
medicare is for ?
old people
medicaid is for?
poor people
CMS
center for medicare and medicaid services
what is stark law
must not refer to lab that owns another lab/who has interests
age discrimination in employment act
ADEA 1967
the perported benefits of co-culture are all of the following except: a: prod of ammonia that stims embryo devel bprod of embryotoxic factors c: rel growth factors into CM c; removal of toxins from medium
A
which of the following may be added to proteins in human serum: q vit/gluc and fatty acids b: gluc, hormones and vit c; fatty acids, glu and hormaones d: hormones, vit and fatty acids
D
glutathione is a marker of what in the oocyte
cytoplasmic maturation
sperm gain potential to fertilize at which stage
during passage through female tract
what are the sequences of events in the egg after fert:
intracellular Ca release-meiotic reactivation-separation of chromatids
preimplantation cleaveg is charcterised by what frequenct
initially slow follwed by more rapid cleavage
implantation is categorized by what events
hatching-apposition-invasion-syncytialization
2 main functions of amniotic fluid are:
shock absorber and buffer temp changes
primary follicle devl involves which changes:
granulosa cell prolif and increase in oocyte vol
LH surge is triggered under what hormonal conditions
high E2 decreasing FSH
how many eggs left at puberty
400,000
how many eggs at birth
2,000,000
how many eggs at menopause
0
primordial follicle is unstimulated
primary follicle has started stim
In an adult human female, the development of a primordial follicle containing an oocyte to a preovulatory follicle takes how long?
in excess of 120 days.
primary oocyte lives in the primordial follicle, is it haploid or diploid
diploid
dictyate or dictyotene
prolonged resting of primary oocyte phase up to 50 years
what do connexin proteins do in oocyte?
gap juction proteins maintain cooyte arrest
what is the muscosal layer of the vag mainly comprised of
stratified squamous eplithelium and no secretory cells
endometrium is in 3 layers. 2 are sensitive to steroid stim and are called stratum functionalis. what are the 2 layers called:
stratum spongiosum and stratum compactum
how many eggs by 5th month of gestation
6-7 million
ratio of ICM to TE cells:
1:3
primordial follicles-primary follicles-secondary follicles what is egg stage in each
primary oocyte-secondary oocyte only at ovulation
is a morula charachterised by a polarized outer cell population?
yes
when does developmental failure occur most often?
3rd meiotic division and blastulation
best indictaor of embryo competency
ICM
aneuploidy
multinucleation
what is a centimorgan
distance between chromosome positions for which there is 1% chance of a crossover
are mitochondrial disorders typically mosaic for the condition
YES
does the presence of h2O2 prod lactobacillus enhance implantation?
YES
Does vit cryoprotectant have high or low MW
HIGH
what is cell lysis caused by during thawing
osmotic stress
FSH
secreted by ant pit in response to GnRH from hypothalamus.
E2
secreted by granulosa cells which use androgens from theca cells to make E2
action of E2
grows follicles and stimulates endo thickening
P4
prod by CL formed after ovulation and then by placenta
hCG
prod by trophectoderm
LH
prod by ant pit. Surge initiates ovulation and occurs in response to high E2 levels
FSH receptors on what cells
granulosa and sertoli cells
sertoli cells do what?
make inhibin (negative feedback) and androgen binding protein
leydig cells produce what?
testosterone in response to LH
hormonal control of spermatogenesis
semen cut off variables for all WHO editions
In the Dr. Menkveld presentation, no WHO 5th vs. WHO 4th Morphology criteria differences are mentioned, only a lower reference limit. Strict criteria is used for morphology assessment and grading in both manuals.
- The concept of normal spermatozoon by WHO 4th and 5th manuals are the same.
- Most of WHO 4th and 5th Morphology criteria are the same.
- There are the slight but not significant differences in some dimensional details that were updated in the WHO 5th manual.
• The WHO 4th manual Morphology reference value was not final and was updated in the WHO 5th edition.
The overall conclusion is that the WHO 4th and 5th Morphology criteria are similar, as they are based on the same strict principles, similar guidelines and reference publications. As the WHO 4th and 5th criteria are similar, we did not conduct any additional study for the WHO 5th morphology algorithm and stayed with the same developed for the WHO 4th. Only the reference value was changed in the V-Sperm.
difference between WHO 4th and 5th
