HASH BROWNS Flashcards
Double embedding: Infiltrated with______ then embedded with______
Celloidin, Paraffin
Flotation water bath:
45 to 50 C
To remove formalin pigments:
Picric acid
Function of alum in hematoxylin:
Mordant
Primary importance of Frozen Sections:
RAPID DIAGNOSIS
To remove mercurial deposits:
Iodine
Explosive when dry:
Picric acid
Enzyme histochemistry:
Frozen section
Second best choice for routine cytologic examination after Papanicolau:
Phase contrast microscopy
NOT SUITABLE for kidney structures:
Bouin’s
Cell death due to ischemia (loss of blood supply) is known as infarction, and is manifested by characteristic histologic appearance:
COAGULATION NECROSIS
Pseudomembranous colitis and diarrhea:
Clostridium difficile
Corynebacterium amycolatum: Most frequently recovered Corynebacterium species from human clinical material. It is part of the___________.
normal skin microbiota
Primary fungal pathogen in HIV patients:
Candida albicans
Double-walled, wrinkled cyst form:
Acanthamoeba castellanii
Intracellular form of blood and tissue flagellates:
leishmanial form
Normal stool pH:
pH 7 to 8
Stool pH associated with CHO disorders:
pH 5.5 or less
Microhematocrit:
10,000 g for 5 minutes
Standing plasma test: _______ (chylomicrons); _______ (VLDL)
creamy layer, turbid
Microanatomical fixatives should ___________ because it inhibits hematoxylin.
never contain osmic acid/osmium tetroxide
Nuclear fixatives should _______ due to its affinity for nuclear chromatin.
contain glacial acetic acid
Cambridge/Rocking microtome: invented by _______
Paldwell Treffall
Bond between Best carmine and glycogen:
Coulombic attraction/electrostatic bonds, hydrogen bonds
Routine H and E: Regressive staining, it involves a ______
differentiation step
Stains for the glomerular basement membrane:
PAS, Azocarmine stain
Postmortem clotting:
immediately after death, rubbery consistency
Antemortem thrombi:
friable, characterized by fibrin precipitation
Leadership:
DIRECTING
COMPONENTS OF FIBRIN GLUE:
cryoprecipitate (fibrinogen) and topical thrombin
Donor deferral, measles (rubeola) vaccination:
2 weeks
Donor deferral, German measles (Rubella) vaccination:
4 weeks
When stained with Sternheimer-Malbin stain, _______ as opposed to the VIOLET COLOR usually seen with NEUTROPHILS.
GLITTER CELLS stain LIGHT BLUE
Second most prevalent protein in CSF:
Prealbumin (transthyretin)
_______ which is usually defined as a newborn’s first bowel movement, is formed in the intestine from fetal intestinal secretions and swallowed amniotic fluid. It is a _____ mucus-like material. It may be present in the amniotic fluid as a result of fetal distress.
MECONIUM, dark green
Blood should NEVER be drawn from a vein in an arm with a ____ (temporary dialysis access device) or ______ (a permanent surgical fusion of a vein and an artery).
cannula, fistula
Adverse reaction of Aminoglycosides:
Nephrotoxicity and ototoxicity
________: neuromotor irritability accompanied by muscular twitching and eventual convulsions; generally due to _______
TETANY, low calcium levels (hypocalcemia)
Reagent for the APT test:
1% NaOH
APT test:
fetal blood, pink solution
APT test:
maternal blood, yellow-brown supernatant
Florence test:
test for choline
Iodine, KI/ dark brown rhombic crystals
Barbiero’s test:
test for spermine
Picric acid, TCA/ yellow leafshaped crystals, needles
Blondheim’s test:
test to differentiate hemoglobin from myoglobin, ammonium sulfate will precipitate hemoglobin
Nanometer is also ______
millimicron
Embedding medium for EM is ______
Plastic
Best vital stain is
neutral red
Vital stain for mitochondria is
Janus Green
Ferning:
Early pregnancy
Pap’s consists of 3 stains:
Harris hematoxylin, OG 6 and EA
Total renal BLOOD flow is
1200 mL/min
Total renal PLASMA flow is
600 to 700 mL/min
Most potent estrogen is
Estradiol
Most important androgen in terms of potency and amount secreted is (Marshall)
testosterone
Conn syndrome:
primary aldosteronism
Hirsutism: male-pattern hair growth in women; most common cause is
PCOS (polycystic ovary syndrome, Marshall)
Primary male hypogonadism
Decreased testosterone
Increased LH and FSH
Secondary male hypogonadism
Decreased testosterone
Decreased LH and FSH