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M&P > hanging drop > Flashcards

hanging drop Flashcards

1
Q

deamination of acetamide results in a ______ color

A

(+) blue
(-) green (no color change)

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2
Q

Acetamide Utilization Test
POS Control
NEG Control

P. aeruginosa
E. faecalis
N. gonorrhea
E.coli

A

POS Control: P. aeruginosa
NEG Control E.coli

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3
Q

Differentiate organism based on their ability to use acetate as a sole source of carbon. Generally differentiates Shigella from E. coli

A

Acetate utilization

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4
Q

POS result for acetate utilization
NEG result

A

(+) blue
(-) no color change or green

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5
Q

Acetate Utilization
POS control
NEG control

Shigella sonnei
S. pneumoniae
E. coli
B. mallei

A

POS control: E. coli
NEG control: Shigella sonnei

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6
Q

Test is used for the presumptive identification of Strep Grp A (S. pyogenes) from other b-hemolytic strep

Staphyloccoccus from Micrococcus

A

Bacitracin (0.04 unit Taxo A)
POS control: S. pyogenes, Microccus luteus
NEG control: S. agalactiae, S aureus

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7
Q

This test differentiates enterococci and group D streptococci from non-group D viridans strep

acetate utilization
bile solubility test
butyrate disk
bile esculin test

A

Bile esculin test

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8
Q

Positive result for bile esculin agar

A

(+) blackening of the slant or medium
brown black precipitate

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9
Q

Bile Esculin Agar
POS control
NEG control

S. agalactiae
E. coli
S. pyogenes
E. faecalis

A

POS control: E. faecalis
NEG control: E. coli
S. pyogenes

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10
Q

differentiates streptococcus pneumoniae from alpha hemolytic streptococci

bile esculin agar
butyrate disk
bile solubility test

A

Bile solubility Test
(+) lysed colonies

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11
Q

Bile solubility test
POS control
NEG control

S. agalactiae
E. coli
S. pneumoniae
E. faecalis

A

POS control: S. pneumoniae
NEG control: E. faecalis

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12
Q

Rapid test to detect enzyme butyrate esterase to aid identification of M. catarrhalis

A

Butyrate Disk
NEG control = N. gonorrhea
POS = M. catarhhalis

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13
Q

POS result for butyrate disk

A

blue w/in 5 mins
indigo to violet

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14
Q

Used to differentiate Grp B strep (S. agalactiae from other streptococcal spp.

L. monocytogenes is also positive

A

CAMP Test

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15
Q

CAMP Test
POS Control
NEG control

S. aureus
S. agalactiae
S. pyogenes
E. coli

A

POS Control: S. agalactiae
NEG control: S. pyogenes

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16
Q

differentiates microccal and staphylococcal species from streptococcal spp. (neg)

A

catalase
(+) bubbles, hydrogen peroxide

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17
Q

Catalase
POS control
NEG control

S. aureus
S. agalactiae
S. pyogenes
E. coli

A

POS control: S. aureus
NEG control: S. pyogenes

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18
Q

Test used to isolate and purify p. aeruginosa

A

cetrimide
(+) growth

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19
Q

Cetrimide
POS control
NEG control

E. faecalis
P. aeruginosa
Providencia
E. coli

A

POS control: P. aeruginosa
NEG control: E. coli

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20
Q

Indicator of citrate test

A

bromthymol blue
(+) blue / growth

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21
Q

Citrate test
POS control
NEG control

E. aerogines
P. aeruginosa
K. pneumoniae
E. coli

A

POS control: E. aerogines, K. pneumoniae
NEG control: E. coli

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22
Q

Coagulase Test
POS control
NEG control

S. epidermidis
E. coli
S. aureus

A

POS control: S. aureus
NEG control: S. epidermidis

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23
Q

Differentiate decarboxylase producing enterbacteriaceae from other gram neg rods

A

decarboxylase test (moeller’s)
LOA
(+) purple
(-) yellow or no color change

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24
Q

POS Control for LOA
Lysine
Ornithine
Arginine

E. aerogenes
K. pneumoniae
E. cloaccae

A

Lysine: K. pneumoniae
Ornithine: E. aerogenes
Arginine: E. cloaccae

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25
Q

NEG Control for LOA
Lysine
Ornithine
Arginine

E. coli
P. vulgaris
C. freundii

A

Lysine: C. freundii
Ornithine: P. vulgaris
Arginine: E. coli

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26
Q

DNA hydrolysis pos result

A

colorless around the test organism

(-) medium remains green = no lysis of dna

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27
Q

DNA Hydrolysis
POS control
NEG control

S. epidermidis
E. coli
S. aureus

A

POS control: S. aureus
NEG control: E. coli

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28
Q

Test for enterbacteriaceae in which a positive result is black precipitate with no fluorescence

A

Esculin hydrolysis
(-) No blackening and fluorescence

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29
Q

Esculin Hydrolysis
POS control
NEG control

S. epidermidis
E. faecalis
E. coli
S. aureus

A

POS control: E. faecalis
NEG control: E. coli

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30
Q

Peptone medium with andrade’s indicator is used to differentiate: ____________

BHI with bromcresol purple indicator is used to differentiate _____________
a. streptococci and enterococci
b. enteric and coryneforms

A

Peptone medium with andrade’s indicator is used to differentiate: B

BHI with bromcresol purple indicator is used to differentiate: A
a. streptococci and enterococci
b. enteric and coryneforms

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31
Q

Peptone medium with andrade’s indicator
POS result
NEG result

A

(+) pink with or without gas
(-) no change in color medium (straw colored)

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32
Q

Andrade’s indicator
POS w/ gas
POS w/o gas

E. coli
S. sonnei
S. flexneri

A

POS w/ gas: E. coli
POS w/o gas: S. flexneri

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33
Q

BHI with bromcresol purple
POS result
NEG result

A

POS result: yellow
NEG result: no color change (purple)

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34
Q

BHI with bromcresol purple
PC
NC

E. coli
M. osteonsis
S. flexneri

A

PC with gas: E. coli
NC: Moraxella osteonsis

35
Q

production of gelatinases capable of hydrolizing gelatin

presumptive for staph, entero and some gram pos bacilli

A

Gelatin Hydrolysis
(+) partial or total liquefaction within 4hrs at 4degrees
(-) complete solidification at 4C

36
Q

Gelatin hydrolysis
PC
NC

E. coli
B. subtilis
B. cepacia

A

PC: B subtilis
NC: E. coli

37
Q

growth at 42C

A

Pseudomonas (PC)
P. fluorescens (NC)

38
Q

Hippurate Test
POS result
NEG result

violet
deep purple
colorless to yellow pink
colorless to purple

A

POS result: deep purple
NEG result: colorless or yellow pink

39
Q

Test used to identify organisms that produce tryptophanase

40
Q

Indole
POS result
NEG result

A

POS result: pink to wine colored ring
NEG result: no color change

41
Q

Indole tests reagents

A

Kovac or Ehrlich

42
Q

Hippurate Test
PC
NC

S. agalactiae
S. aureus
S. maltophilia
S. pyogenes

A

PC: S. agalactiae
NC: S. pyogenes

43
Q

Indole
PC for Kovacs:
PC for ehrlich:

E. coli
H. aegypticus
H. influenzae
E. tarda

A

PC for Kovacs: E. coli
PC for ehrlich: H. influenzae

44
Q

test that identifies catalase negative gram positive cocci using the cinnamaldehyde reagent

Litmus Milk
LAP
Hippurate
Andrade’s

A

LAP
(+) red color (leucine aminopeptidase)

45
Q

LAP Test
PC
NC

E. coli
E. faecalis
A. virdans

A

PC: E. faecalis
NC: Aerococcus viridans

46
Q

Litmus Milk Medium
fermentation - gas production

A. P. aeruginosa
B. L. acidophillus
C. C perfringens

47
Q

Litmus Milk Medium
Acid - clot formation

A. P. aeruginosa
B. L. acidophillus
C. C perfringens

48
Q

Litmus Milk Medium
Peptonization - clearing

A. P. aeruginosa
B. L. acidophillus
C. C perfringens

49
Q

bright red color indicative of mixed acid fermentation

50
Q

red color indicative of acetoin production

51
Q

negative result for MRVP

52
Q

MR POS, VP NEG control
MR NEG, VP POS control

E. coli
S. sonnei
E. aerogenes
K. pneumoniae

A

MR POS, VP NEG control = E. coli
MR NEG, VP POS control = E. aerogenes

53
Q

This test differentiates gram pos. cat pos cocci

oxidase
microdase

54
Q

Positive result for microdase test

A

blue to purple blue color

55
Q

Microdase Test
PC
NC

S. aureus
E. coli
M. luteus

A

PC: M. luteus
NC: S. aureus

56
Q

Semisolid Agar deep - for motility
POS result
NEG result

A

POS result: organisms spread from site of inoculation
NEG result: non-motile, remain at site of inoculation

57
Q

PC for hanging drop and semisolid agar deep

58
Q

Used to determine if organism forms gas during glucose fermentation. Some lactobacillus and leuconostoc produce gas

A

MRS broth
(+) gas production

59
Q

MRS broth
Growth, gas production:
Growth, no gas:

A

Growth, gas production: leuconostoc
Growth, no gas: lactobacillus

60
Q

Identifies various genera of enterobateriaceae and verotoxin producing E. coli

A

MUG Test
(+) electric blue fluorescence

61
Q

MUG Test
PC
NC

E. faecalis
E. coli
K. oxytica
K. pneumoniae

A

PC: E. coli
NC: K. pneumoniae

62
Q

Determine whether an organism can reduce nitrite to gaseous nitrogen

A

Nitrite Reduction
(+) no color change within 2 mins
(-) red

63
Q

Nitrite Reduction
PC
NC

P. vulgaris
P. mirabilis
A. baumanii
E. coli

A

PC: P. mirabilis
NC: A. baumanii

64
Q

Tests whether an organism can produce B-galactosidase

65
Q

Positive Result for ONPG
blue
green
yellow
pink

A

yellow
(-) colorless

66
Q

ONPG
PC
NC

S. pneumoniae
S. sonnei
S. typhimurium

A

PC: S. sonnei
NC: S. typhimurium

67
Q

Positive result for optochin (zoi)

A

14mm or greater ZOI

68
Q

Optochin
PC
NC

S. pneumoniae
S. sonnei
S. pyogenes
S. typhimurium

A

PC: S. pneumoniae
NC: S. pyogenes

69
Q

Positive result of oxidase test

A

dark purple color within 10 secs

70
Q

Oxidase Test
PC
NC

S. pyogenes
S. aeruginosa
S. typhimurium
E. coli

A

PC: S. aeruginosa
NC: E. coli

71
Q

Reagent of Phenyalalanine Deaminase

A

ferric chloride

72
Q

Phenyalanine Deaminase
PC
NC

P. mirabilis
P. vulgaris
E. coli

A

PC: P. mirabilis
NC: E. coli

73
Q

Test that differentiates group A streptococci and enterococci by the presence of L-pyrolidonyl arylamidase

A

PYR test
(+) bright red color, cinamaldehyde reagent

74
Q

PYR Test PC (2)

S. pyogenes
E. coli
E. faecalis
S. aureus

A

S. pyogenes
E. faecalis

75
Q

Differentiates E. faecalis (pos) and E. faecium (negative)

PYR
Pyruvate broth
Spot Indole

A

Pyruvate broth

76
Q

Positive result for pyruvate

Blue to green
Green to yellow
Yellow to blue

A

green to yellow

77
Q

PC control for pyruvate broth and salt tolerance

A

E. faecalis

78
Q

NC for salt tolerance

79
Q

Positive result for salt tolerane

A

turbidity
purple to yellow

80
Q

Determine the presence of the enzyme tryptophanase

A

Spot indole
(+) blue

81
Q

PC for spot indole

82
Q

Proteus may be presumptively identified by the ability to hydrolyze ______

83
Q

Urea Hyrdrolysis Christensen’s method
POS result
NEG result

A

POS result: light orange to magenta
NEG result: no color change

84
Q

Urea Hydrolysis
PC
NC

P. vulgaris
K. pneumoniae
E. coli

A

PC: P. vulgaris
Weakly Pos: K. pneumoniae
NC: E. coli

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