Handling Of Specimens

Question 1

defined as a representative sample of a tissue body fluid or other site that is analyzed in the clinical laboratory

Mas represent the actual infected site and does must be collected from the site of the infection

Answer 1

SPECIMEN

Question 2

+Use for the removal of residual antibiotics in blood specimens +bcb contains resin that inactivates antibiotics

Answer 2

Antibiotic Removal Device{ARD}

Question 3

Inadequate specimens should be marked

Answer 3

QNS

Question 4

Gastroenteritis

Answer 4

Acute/early phase of diagnosis

Question 5

Viral Meningitis

Answer 5

2-3 days after onnset of symptoms

Question 6

Typhoid Fever

Answer 6

Week 1 blood. B
Week 2 Urine U
Week 3 Stool. S
Week 4 Antibody A

Question 7

Specimen should be collected in sterile containers except for____ which can be collected in clean leak proof containers

Answer 7

STOOL

Question 8

Swabs are not recommended for collection because

Answer 8

+Do not provide sufficient
+quantity are easily contaminated
+are prone to drying
+never appropriate for an aerobic culture because the atmospheric oxygen is toxic to anaerobics

Question 9

Lesions wounds and abscesses should be collected from the advancing margin preferably by__ rather than swabs

Answer 9

Needle Aspiration

Question 10

Is an order form that is sent to laboratory along with the specimen

Answer 10

Specimen Requisition

Question 11

Specimen transport

Answer 11

Specimen should be transported to laboratory immediately with in 30 minutes and not longer than 2 hours after collection

Question 12

Specimen transport

Answer 12

For an aerobic bacteria that transport should not take more than 10 minutes

For sterile sample such as csf and body fluids preferably 15 minutes after collection

Question 13

Air transport. IATA and CAO

Answer 13

International Air Transport Association and Civil Aviation Organization

Question 14

Maintains the appropriate colony counts at room for 24 hours use in urine sample

Answer 14

Boric Acid

Question 15

Maintains integrity of tropozoids and cease examination

Should not be added on stool for bacteriological testing

Answer 15

Polyvinyl alcohol and buffered formalin

Question 16

This organism should be collected without preservative

Answer 16

Clostridium difficile

Question 17

Used to preserve clotting of specimens such as blood bone marrow and synovial fluid because microorganism will otherwise be bound in the clot

Answer 17

Anticoagulants

Question 18

Commonly used anticoagulant for varial cultures and mycobacterium species.

May inhibit growth of gram positive bacteria and his

Answer 18

Heparin

Question 19

Most common anticoagulant used for microbiology specimens

Anti complementary and anti phagocytic properties

May inhibit the growth of mycoplasma species, niserria species, gardnerla vaginalis,streptobacillus moliniformis, pepto streptococcus anaerobius

Answer 19

0.025% to 0.05% sodium polyanethol sulfonate

Question 20

Citrate, ethylene diamine tetraacetic acid, acid citrate dextrose or other anticoagulants

Answer 20

should not be used for microbiology because they may suppress the growth of organisms

Question 21

Has structural homology with sps but is less effective in neutralizing serum bacteriacidal activity and his inhibitory property to GNB

Answer 21

Sodium amylosulfate (SAS)

Question 22

Sustain the viability of microorganisms present in a specimen but do not allow further multiplication

Answer 22

Transport media

Question 23

is added to this media to absorb fatty acids present in specimens that could result in ph changes in the media and the killing of fastidious organisms such as nigeria gonorrhea and bordetella pertusis

Answer 23

Charcoal

Question 24

PRAS

Answer 24

Pre-reduces anaerobic sterilize

Question 25

JEMBEC

Answer 25

John E. Martin Biological Environment Chamber

Contain selective agar cap and the carbon dioxide co2 generating tablet

Commercial transport system used for neisseria gonorrhea specimen

Question 26

Cary blair medium

Answer 26

Stool pathogen

Question 27

Stuart medium

Answer 27

Multipurpose transport media
also to isolate viruses

Question 28

Amies Medium

Answer 28

For Respiratory sample

Question 29

Morified Todd Hewitt

Answer 29

For streptococcus a galact j from vaginal swabbing

Question 30

Hanks balance salt solution

Answer 30

With bovine albumin and stuarts transport media

Question 31

Leibovitis emory media

Answer 31

Viral transport like Hanks and Stuart

Question 32

Transgrow

Answer 32

Good for nigeria species and bordetella pertussis

Question 33

Viral transport medium

Answer 33

It is ideal for respiratory and gentle samples i and you discharge tissue and skin specimens intended for molecular test

Gentamicin and amphotericin b are present to suppress the growth and indigenous flora

Maintains the viability of the organism for 48 hours at 2 to 8 degrees celsius and even at room temperature while on storage requires negative 70° c

Question 34

Specimen preservations

Answer 34

Jembeck
Carry blair medium
Stuart medium
Amies medium
Modified todd-hewitt
Hanks balance salt solution
leibvitis emery media
Transgrow
Viral transport medium

Question 35

Recommend skin disinfectant for babies 2 months + and has allergies to iodine

Answer 35

Chlorhexidine gluconate