Hand Washing Lab

Question 1

Normal Flora

Answer 1

• Staphylococcus epidermidis
• Corynebacteria
• Staphylococcus aureus (also transient)
• Propionibacterium
• Streptococcus pyogenes
• Candida albicans (yeast)

Question 2

Group A Streptococci

Answer 2

strep throat

Question 3

Staphylococcus aureus

Answer 3

acne

Question 4

Klebsiella

Answer 4

wound infections

Question 5

Clostridium difficile

Answer 5

colitis

Question 6

Haemophilus

Answer 6

pink eye

Question 7

Pseudomonas aeruginosa

Answer 7

wound infection (blue-green pus)

Question 8

E. coli

Answer 8

diarrhea and/or uti

Question 9

Bacteroides

Answer 9

ear infection or vaginitis (after BM and not washing hands)

Question 10

Shigella

Answer 10

diarrhea

Question 11

Haemophilus influenzae

Answer 11

pneumonia

Question 12

Bacillus species

Answer 12

regular contaminant from soil

Question 13

What is the purpose of washing hands?

Answer 13

To prevent the spread of disease

Question 14

What is the purpose of the normal flora on our skin?

Answer 14

To protect us from the invasion of pathogens

Question 15

3 groups of normal flora

Answer 15

1. Diptheroids
2. Staphylococci
3. Yeast and Fungi

Question 16

Diptheroids

Answer 16

• gram positive
• non-pathogenic
• Propionibacterium acnes > in pores and hair follicles - breaks down oily substances

Question 17

Staphylococci

Answer 17

• inhibit pathogens from establishing any presence on skin

- Staph epidermidis

Question 18

Yeast and Fungi

Answer 18

degrade lipid secretions

Question 19

Transient Bacteria

Answer 19

• bacteria we pick up from environment
• temporary contaminants
• easily removed

Question 20

Hand Sanitizer

Answer 20

• 65% ethanol
• dries bacteria from the inside out
• coagulates proteins

Question 21

Alcohol-Free Sanitizer (Wet Ones)

Answer 21

• 0.3% benzalkonium chloride

- targets cell membrane

Question 22

Povidine iodine scrubs

Answer 22

• 13% iodine
• iodine crystals “pop” bacteria
• bacteria ruptures and dies

Question 23

Antibacterial soap

Answer 23

0.3% chloroxylenol

Question 24

Kirby Bauer Disk of Diffusion Method

Answer 24

-tests sensitivity of antibiotics and disinfectants

Question 25

Antimicrobial

Answer 25

inhibits or kills bacteria or other microbes

Question 26

Antibiotic

Answer 26

• kills bacteria
• produced by other microbes to inhibit or kill other types of microbes
• inhibit cellular processes

Question 27

Disinfectants and antiseptics

Answer 27

• chemical agents that inhibit microbial growth
• disinfectants are used on inanimate objects and can kill spores
• antiseptics can be used on human tissue and cannot kill spores

Question 28

Bacteriotatic

Answer 28

inhibits growth of bacteria

Question 29

Bacteriocidal

Answer 29

kills bacteria

Question 30

Zone of Inhibition varies based on…

Answer 30

• diffusability of agent
• size of inoculum (how much bacteria)
• type of medium used