Haemostasis and Bleeding Disorders Flashcards
- List some pro-coagulant factors in the body.
Platelets
Endothelium
vWF
Coagulation cascade
- List some anti-coagulant factors in the body.
Fibrinolysis
Anti-thrombins
Protein C/S
Tissue factor pathway inhibitor
- Which three responses are stimulated by vessel injury?
Vasoconstriction Platelet activation (forms primary haemostatic plug) Activation of the coagulation cascade
- What are the components of blood clot formation?
Vascular endothelium
Platelets
Coagulation factors
White blood cells
- What are the two main functions of the endothelium?
Synthesis of prostacyclin, vWF, plasminogen activators and thrombomodulin
Maintain barrier between blood and pro-coagulant subendothelial structures
- How many platelets are produced by each megakaryocyte?
4000
- What is the life span of platelets?
10 days
NOTE: this is important because it means that the effect of antiplatelet drugs lasts for 10 days after stopping the drug
- What are glycoproteins?
Cell surface proteins through which platelets can interact with the endothelium, vWF and other platelets
- What do dense granules contain?
Energy stores (ATP and ADP)
- Which features of platelets enable them to massively expand their surface area?
Open cannalicular system and microtubules and actomyosin
- What are the two ways in which platelets can adhere to sub-endothelial structures?
DIRECTLY – via GlpIa
INDIRECTLY – via binding of GlpIb to vWF (this is MORE IMPORTANT)
- Which factors, released by platelets after adhesion, promote platelet aggregation?
ADP
Thromboxane A2
- How do platelets bind to each other?
GlpIIb/IIIa
It also binds to fibrinogen via this receptor
- Describe the effects of aspirin and other NSAIDs on the arachidonic acid pathway.
Aspirin is an irreversible COX inhibitor
Other NSAIDs reversibly inhibit COX
- What is the rate-limiting step for fibrin formation?
Factor 10a
- What are the effects of thrombin?
Activates fibrinogen
Activates platelets
Activates profactors (factor 5 and 8)
Activates zymogens (factor 7, 11 and 13)
- Name the complex that is responsible for activating prothrombin to thrombin.
Prothrombinase complex
- Outline the initiation phase of the clotting cascade.
Damage to the endothelium results in exposure of tissue factor which binds to factor 7 and activates it to factor 7a
The tissue factor-factor 7a complex then activates factors 9 and 10
Factor 10a binds to factor 5a resulting in the first step of the coagulation cascade
- Outline the amplification phase of the clotting cascade.
Activated factors 5 and 10 will result in the production of a small amount of thrombin
This thrombin will activate platelets
Thrombin will also activate factor 11 which activates factor 9
Thrombin also activates factor 8 and recruits more factor 5a
Factors 5a, 8a and 9a will bind to the activated platelet
- Outline the propagation phase of the clotting cascade.
Activated factors 5, 8 and 9 will recruit factor 10a
This results in the generation of a large amount of thrombin (thrombin burst)
This enables the formation of a stable fibrin clot
- Why is the prothrombinase complex important?
It allows activation of prothrombin at a much faster rate
- What is required for adequate production/absorption of vitamin K?
Bacteria in the gut produce vitamin K
It is fat-soluble so bile is needed for vitamin K to be absorbed
- What is the most common cause of vitamin K deficiency?
Warfarin
- Name two factors that convert plasminogen to plasmin.
Tissue plasminogen activator
Urokinase
- Name a factor that inhibits the factors mentioned in question 24.
Plasminogen activator inhibitor 1 and 2
- Name two factors that directly inhibit plasmin.
Alpha-2 antiplasmin
Alpha-2 macroglobulin
- What is the role of thrombin-activatable fibrinolysis inhibitor (TAFI)?
Inhibitor of fibrin breakdown