H/O RC Memorize

Question 1

B ALL immunophenotype

Answer 1

CD10+ (cALLA)+, CD19+, CD22+, CD79a+, TDT+, HLA-DR+

Question 2

T ALL immunophenotype

Answer 2

cCD3+, Most sCD3+ and TdT+, often CD2+, CD4+, CD7+, CD8+, Variable CD10+ Neg HLA-DR-

Question 3

ETP ALL immunophenotype?

Answer 3

cCD3+, CD7+, Weak CD5+, CD1a-, CD8-

One or more positive in at least 25% of lymphoblasts: CD117+, CD34+, HLA-DR+, CD13+, CD33+, CD11b+

Question 4

Infant ALL immunophenotype?

Answer 4

CD10- (unless germline KMT2A-r which is CD10+)

Question 5

Most common B ALL translocation (~28%)?

Answer 5

ETV6-RUNX1 (aka TEL-AML1)

t(12;21) (p13;q22) (cryptic)

Question 6

B ALL neutral translocation with high incidence of CNS disease and CNS relapse, high WBC?

Answer 6

TCF3-PBX1 (aka E2A-PBX1)

t(1;19)

Question 7

B ALL with profound eosinophilia, lower blast count?

Answer 7

IL3-IGH

t(5;14)

Question 8

Phillidelphia? Most common age?

Answer 8

BCR-ABL1 (Ph+) PCR p190
t(9;22)
Teens

Question 9

Ph-like? potential TKIs? All are associated with ____.

Answer 9

CRLF2 (50%) - t(X;14) - +/- JAK2 or JAK1 point mutations - ruxolitinib
JAK2 or EPOR (15-20%) - ruxolitinib
ABL class - ABL1, ABL2, CSF1R
PDGFRB (tY;14) (10-15%) - dasatinib/imatinib
All are associated with constitutive activation of kinase signaling

Question 10

Translocation in 75% of infant ALL?

Answer 10

KMT2A (aka MLL, 11q23)

t(4;11), t(9;11)

Question 11

B ALL, poor prognosis, older age, lower WBC, high relapse rate?

Answer 11

iAMP21

Question 12

Ikaros? Population?

Answer 12

IKZF1

Mexican

Question 13

DNA index for hyperdiploid ALL? how many chromosomes? Best trisomies?

Answer 13

DNA index >1.16
OR 53+ chromosomes
Trisomies 4, 10

Question 14

DNA index for hypodiploid?

Answer 14

DNA index <0.81 or <44 chromosomes

Fewer chromosomes - worse prognosis

Question 15

T ALL?

Answer 15

• Few with prognostic significance
• t(11;14)(p13;q11.2)
• TCRdelta 14q11
• TCRbeta 7q35
• NOTCH1

Question 16

Definitions of M1, M2 and M3 marrow?

Answer 16

M1: <5% blasts
M2: 5-25% blasts
M3: >25% blasts

Question 17

Burkitt leukemia?

Answer 17

t(2;8)(p12;q24)

c-Myc to an Ig heavy or light chain gene