GWTF ED

Question 1

Ussing Chamber

Answer 1

passing of molecules across the membrane - transepithelial resistance

Question 2

Processes for isolated tissue

Answer 2

Smooth muscle
Force transducer
Physiological conditions
Measure dose response curve
control, wash, repeats
efficacy and potency

Question 3

analysis of relaxation?

Answer 3

pre-contraction: carbachol on detrusor, NA on urethra SM

Question 4

carbachol

Answer 4

ACh agonist

Question 5

isoprenaline

Answer 5

broad-spectrum B-adrenergic agonist

Question 6

atropine

Answer 6

broad-spectrum muscarinic blocker/antagonist

Question 7

processes for identifying a receptor

Answer 7

Q PCR
western blot
immunocytochemistry

Question 8

quantitative PCR

Answer 8

shows mRNA expression, gene repeat

Question 9

western blot

Answer 9

confirms the expression of the gene to produce the protein

Question 10

immunocytochemistry

Answer 10

expressed in the membrane?

Question 11

force transducer

Answer 11

used to measure the force of contraction produced by an isolated tissue preparation

Question 12

potency

Answer 12

the concentration required to produce a given effect

Question 13

efficacy

Answer 13

magnitude of the effect

Question 14

EC50

Answer 14

the concentration required to produce half the maximum response

Question 15

IC50

Answer 15

concentration of an inhibitor that reduces the maximal response by half

Question 16

whole cell voltage clamp

Answer 16

can generate IV graphs
holding potential and stepping
add blockers, look for differences in traces

Question 17

study of axonal connections

Answer 17

pseudorabies virus - can cross synapses

retrograde