Growth Kinetics

Question 1

First 2 phases of Bacterial Growth?

Answer 1

Lag: No macroscopic increase in cell population. Attempt to minimize loss. (To minimize use large inoculum and medium)

Exponential: Occurs when cell population is NOT limited. Characterized by doubling time.

Question 2

Last 2 phases of bacterial growth?

Answer 2

Stationary: Number of bacteria being produced = # of bacteria dying (dynamic condition)

Death: Cell lysis, nutrients run out, cells present but not viable

Question 3

How is cell number monitored?

Answer 3

Microscopic count

Viable cell count (petri dish)

Question 4

How is cell massed measured?

Answer 4

Cell dry weight (centrifugation)

Turbidity (optical density)

Question 5

3 types of inhibition?

Answer 5

Competitive: Inhibitors and substrates are competing for the same active site
Uncompetitive: No affinity to enzyme itself but bind to the complex reducing effectiveness.
Noncompetitive: Bind on sites other than active site and reduce enzyme affinity to the substance.

Question 6

Fermentation Patterns?

Answer 6

Type l: Product is associated with cell growth (overall yields = instantaneous yield)
Type ll: Instantaneous ≠ overall
Type lll: non growth associated (secondary metabolites)