Group1 Flashcards
1
Q
principle causes of ESRD (2)
A
44% diabetes, 27% HTN
2
Q
glomerular barrier parts (3)
A
fenestrated capillary endothelium, basement membrane, podocyte foot processes with slit diaphragms made up of nephrin
3
Q
normal urine proteins (amount, % of two major) and what level of proteinuria is nephrotic?
A
Normal urine has < 150 mg/day proteins: 45% Tamm Horsfall (secreted by TAL epithelium), 35% albumin (escapes reabsorption in PCT); if urine has more than 3.5 g/day it must be glomerular in origin
4
Q
selective proteinuria: urine findings, mechanism, pathophys
A
pathophys: minimal change disease; mechanism: foot process effacement (loss of charge barrier?); urine: albumin, transferrin, no IgG (too big)
5
Q
non-selective proteinuria
A
pathophys: most glomerulopathy other than min change disease; mechanism: foot process effacement (loss of size barrier?); urine: albumin, transferrin, IgG
6
Q
proximal tubular dysfn proteinuria: urine findings, mechanism, pathophys
A
mech: decr reabs of filtered protein; pathophys: tubulo-interstitial dis, Fanconi; urine: <3 g day proteinuria (B2 microglobulin, lysozyme, albumin) along w/ other signs of PCT dysfn (glucose, bicarb, AA, etc.)
7
Q
overload proteinuria: urine findings, mechanism, pathophys
A
pathophys: plasma cell dyscrasias (multiple myeloma); mech: incr # small, abnormal filterable serum proteins, these proteins can cause PCT toxicity/cast formation (prevent reabs of albumin); urine: <3 g day (non-glomelular), normal urine dipstick for protein (altho poss incr albumin), lots of IgG light chains (Bence Jones protein)
8
Q
orthostatic proteinuria: urine findings, pathophys
A
pathophy: not pathological (seen in healthy teens/young adults); <2 g day proteinuria that appears in first morning urine when pt becomes upright
9
Q
functional proteinuria
A
not pathological, seen in pts w/ high fever, CHF, cold exposure, resolves w/ resolution of precipitating event
10
Q
best method for long term follow-up of proteinuria
A
spot urine protein:creatinine ratio
11
Q
spot urine protein:creatinine ratio
A
best method for long-term follow-up; detects all protein; Upr/Ucr ~ 24 hr protein excretion (nl <.15) b/c Ucr ~ 1000 mg/day; this underestimates Upr in muscular men and overestimates Upr in elderly pts, but still useful for tracking vs time b/c Ucr shouldn’t change
12
Q
nephrotic syndrome
A
non-inflammatory glomerulopathy (seen in CKD); syndrome: glomerular proteinuria (>3.5 g/day), hypoproteinemia, edema, hyperlipidemia
13
Q
diff dx of edema (5)
A
proteinuria, renal failure, CHF, liver failure, protein malnutrition
14
Q
complications of nephrotic syndrome (3)
A
hypercoagulability (due to loss of antithrombin proteins), infection, atherogenesis
15
Q
histopathologies of nephrotic syndrome (3)
A
minimal change disease; focal segmental glomerulosclerosis; membranous glomerulopathy
16
Q
mechs of injury in nephrotic syndrome (3)
A
minimal change -> unclear (T/B mediated); FSGS -> podocyte injury; MGN -> in-situ immune complex to some antigen on podocytes
17
Q
minimal change disease: type of what, LM/FM/EM, etiology, demographics, tx (2), prognosis (3)
A
type of histopath in nephrotic syndrome; normal cx on LM, negative FM, but EM shows foot process effacement; etiology either primary (poss B/T cell mediated) or secondary (NSAIDs, lymphoma); seen mostly in kids; tx w/ corticosteroids for 6 weeks (90-95% remission) -> may give kids steroid trial rather than biopsy if they have nephrotic syndrome w/o HTN and hematuria and w/ normal GFR and C3; 33% never relapse after tx, 33% occasionally relapse, and 33% frequently relapse (>3 in 6 mos)
18
Q
FSGS: type of what, LM/FM/EM, pathogenesis, FSGS factor, demographics (2)
A
type of histopath in nephrotic syndrome; LM has focal (some glomeruli) segmental (parts of these glomeruli) scleroris (too much ECM) w/ segmental collapse w/ adhesions to Bowman’s capsule, FM has IgM and C3 in mesangium of collapsed segments, EM has foot process effacement, focal podocyte injury or denudation of GBM, collapse of cap loops w/ accumulation of hyalin; pathogenesis: final common pathway for glom injury, due to podocyte injury w/ loss of podocytes in urine -> segmental sclerosis; may be due to uPAR (circulating factor, explains why transplanted kidneys can get FSGS in FSGS pts); common in elderly (infantile FSGS is monogenetic)
19
Q
uPAR
A
circulating FSGS factor (explains why pts w/ FSGS can get FSGS in donated kidneys)
20
Q
what disease has no findings on LM or FM but has foot process effacement on EM?
A
minimal change disease
21
Q
what disease is assoc w/ injury and loss of podocytes?
A
FSGS
22
Q
what disease has FM of IgM and C3 in mesangium?
A
FSGS
23
Q
what disease has EM of foot process effacement, focal podocyte injury or denudation of GBM, collapse of capillary loops w/ accumulation of hyalin
A
FSGS
24
Q
what nephrotic syndrome is common in kids?
A
minimal change disease
25
what nephrotic syndrome is common in elderly?
FSGS
26
when do we try a steroid trial?
kids w/ nephrotic syndrome w/o HTN and hematuria and w/ normal GFR and C3 -> way to avoid biopsy if we believe they may have minimal change disease
27
membranous glomerulopathy: type of, LM/FM/EM, etiology (7), natural hx (3)
type of histopath in nephrotic syndrome; LM: thickened glomerular cap loops (thickened BM) w/ BM spikes seen on PAS and subepithelial deposits seen in trichrome, FM: IgG and C3 in granular cap loop deposits (secondary MGN has both cap loop and mesangial deposits of more proteins), EM: foot process effacement, subepithelial electron dense deposits w/ variable amounts of BM surrounding them, secondary MGN may have subendothelial and mesangial deposits too; primary MGN: subepithelial immune complexes form in situ against podocyte membrane antigen (assoc w/ HLA types); secondary: infx (hep B/C, malaria), neoplasms (lung, colon, gastric, breast), meds (gold salts, penicillinamine), SLE; natural hx: 20% spontaneous remission, 60% persistent proteinuria w/o GFR decline; 20% progressive w/ GFR decline
28
etiology of MGN
primary MGN: subepithelial immune complexes form in situ against podocyte membrane antigen (assoc w/ HLA types); secondary: infx (hep B/C, malaria), neoplasms (lung, colon, gastric, breast), meds (gold salts, penicillinamine), SLE
29
what disease can be caused by hep B/C?
MGN
30
what disease can be caused by HIV?
FSGS
31
what disease can be caused by malaria?
MGN
32
what disease can be caused by gold salts?
MGN
33
what disease can be caused by penicillamine?
MGN
34
what disease can be caused by neoplasm?
lung/colon/gastric/breast cancer -> MGN; lymphoma -> minimal change disease
35
what disease can be caused by NSAIDS?
minimal change disease and interstitial nephritis; NSAIDS also cause GFR decline (no afferent dilation)
36
what disease has LM of: thickened glomerular cap loops w/ BM spikes seen on PAS and subepithelial deposits seen in trichrome
MGN
37
what disease of EM of: foot process effacement, subepithelial electron dense deposits w/ variable amounts of BM surrounding them
MGN
38
what disease has FM of: IgG and C3 in granular cap loop deposits
MGN (can have mesangial and other staining in secondary MGN), post-strep (w/ mesangial deposits too), lupus (w/ mesangial deposits and positive w/ other stains too)
39
FM in nephrotic syndromes
minimal change disease: no staining; FSGS: IgM/C3 in mesangium; MGN: IgG/C3 in granular cap loop deposits (secondary MGN can have more proteins in granular loop and mesangial deposits)
40
hematuria/proteinuria differential dx
glomerular disease likely if hematuria and proteinuria (either inflammatory glomerulonephritis aka nephritic syndrome or diabetic nephropathy or heriditary GBM disease); non-glomerular disease likely if hematuria only (if >40 yo, consider cancer, stones, infx; if <40 yo, consider stones, infx, trauma); nephrotic syndrome likely if proteinuria only
41
nephritic syndrome
inflammatory glomerulonephritis (seen in acute and subacute renal failure); syndrome: microscopic hematuria (RBC casts, dysmorphic RBCs), proteinuria (not as high as nephrotic syndrome), HTN, edema
42
nephritic syndrome mnemonic
PHARAOH = Proteinuria, Hematuria, Azotemia, RBC casts, Anti-strep titres (if post-strep), Oliguria, Hypertension
43
nephritic vs nephrotic
nephritic is inflammatory, acute, has hematuria (dysmorphic RBC/RBC casts) and mild proteinuria (3.5 g proteinuria w/o hematuria; nephritic assoc w/ HTN; nephrotic assoc w/ hyperlipidemia and hypoalbuminemia; both have edema (nephrotic due to hypoalbuminemia, nephritic due to Na retention b/c nephritic assoc. w AKI)
44
Rapidly Progressive Glomerulonephritis
2 nested clinical syndromes: subacute rise in Pcr w/ nephritic syndrome
45
pathophys of nephritic syndrome (5 events and timeline)
Capillary wall injury -> proliferation w/in 24 hrs (influx of immune cells to glomerular tuft, not prolif of endogenous glom. cells = hypercellularity on LM) -> acellular crescent formation (4 days, made up of fibrinogen) -> cellular crescent formation (7 days, immune cells and fibroblasts gel onto acellular crescent)-> glomerular and interstitial scarring by fibroblasts (30 days -> irreversible nephron loss)
46
nephritic syndrome diff dx
antibody mediated (anti GBM/Goodpasture's); immune complex mediated (post-strep, lupus, IgA/HSP); pauci-immune glomerulonephritis (ANCA positive small vessel vasculitis)
47
antibody mediated nephritic syndrome etiology
IgG against type IV collagen in GBM
48
Goodpasture's syndrome: type of what, what is it, how does it present, LM/FM/GM,tx (3)
antibody-mediated nephritic syndrome; anti-GBM (IgG against type IV collagen) pulmonary-renal syndrome: presents w/ RPGN and hemoptysis; LM: hypercellular glomeruli w/ fibrin and crescents in Bowman's space; FM: linear GBM staining w/ IgG and C3 w/ stringy fibrin staining in Bowman's space; EM: compressed glomerular tuft, fibrin in Bowman's space, no deposits; tx: plasma exchange, immunosuppression, rituximab (anti CD20)
49
FM in: minimal change disease, FSGS, MGN, anti-GBM, post-strep, lupus, HSP, vasculitis
minimal change dis: no FM staining (LM also normal); FSGS: IgM and C3 in mesangium; MGN: IgG and C3 in granular cap loop deposits; anti-GBM: linear cap loop staining of IgG and C3 w/ stringy fibrin in Bowman's space; post-strep: granular cap loop and mesangial IgG and C3; lupus: granular cap loop and mesangial "full house" deposits (IgG, IgA, IgM, C1q, C4, C3); HSP: mesangial IgA and C3; vasculitis: no FM staining (despite inflamm infiltrate on LM)
50
what disease has normal LM?
minimal change disease
51
what disease has nothing staining on FM?
minimal change disease, vasculitis (altho MCD has nothing on LM while vasculitis has inflammation of LM)
52
what disease has FM: linear cap loop staining of IgG and C3
anti-GBM (also has stringy fibrin in Bowman's)
53
which disease has FM: granular cap loop and mesangial IgG and C3
post-strep GN; lupus (also has more positive stains); MGN has cap loop IgG/C3 but no mesangial unless secondary MGN)
54
which disease has FM: granular cap loop and mesangial "full house" deposits (IgG, IgA, IgM, C1q, C4, C3)
lupus
55
which disease has FM: mesangial IgA and C3
Henoch Schoenlein Purpura (IgA nephropathy variant)
56
what disease has LM: hypercellular glomeruli w/ fibrin and crescents in Bowman's space
anti-GBM -> no PNMs like in post-strep
57
what disease has EM: compressed glomerular tuft, fibrin in Bowman's space, no deposits
anti-GBM
58
post-strep GN: type of what, presentation, LM/FM/EM, tx
type of immune complex mediated nephritic syndrome; presents 21 days post-infx w/ RPGN; LM: diffuse hypercellularity w/ LOTS of PNMs (specific feature), accentuation of lobar architecture, occasional subepi deposits; FM: granular cap loop and mesangial IgG and C3; EM: large discrete subepi deposits w/o sclerotic reaction (diagnostic), subendo and mesangial deposits present but not diagnostic; tx: supportive care (self-limited)
59
what disease has LM: hypercellular glomeruli w/ PNMs, accentuation of lobar architecture, occasional subepi deposits
post-strep GN (PNMs are specific)
60
what disease has EM: large discrete subepi deposits w/o sclerotic reaction, also subendo and mesangial deposits
post-strep GN (subepi deposits diagnostic)
61
lupus: type of what, serology (3), LM/FM, tx (2)
type of immune complex mediated nephritic syndrome; serology: ANA, anti-dsDNA, hypocomplementemia; LM variable: can be prolif GM limited to mesangium, severe diffuse prolif GN similar to post-infx but w/ fewer PNMs, or combo of these w/ MGN, often see "wire loops" due to thickening of cap loops and extensive subendo or subepi deposits; FM: full house granular cap and mesangial deposits; tx: immunosuppression (achieves remission but w/ freq relapses), poss ACEI/ARB for BP control
62
hypocomplementemia seen in?
lupus, post-infectious
63
what has LM with "wire loops"?
lupus
64
subendo deposits seen in
lupus (extensive), secondary MGN (w/ subepi and mesangial)
65
subepi deposits seen in
lupus (extensive), post-strep (large, discrete), MGN (with variable BM surrounding)
66
Henoch Schoenlein Purpura: type of what, presents (4), demographics, LM/FM/EM, Tx
type of IgA nephropathy (immune-mediated nephritic syndrome); presents w/ systemic vasculitis (RPGN, purpuric skin rash, colitis, arthritis); presents in childhood; LM: mesangial hypercellularity, focal cellular crescents; FM: mesangial IgA and C3 deposits; EM: mesangial electron dense deposits; Tx: us. supportive, poss immunosuppresion
67
what disease has LM: mesangial hypercellularity, focal cellular crescents
HSP
68
pauci-immune glomerulonephritis
aka "mechanism X" form of nephritic syndrome; LM has inflammatory infiltrate altho FM shows no staining
69
ANCA positive small vessel vasculitis: aka (2), type of what, LM/FM/EM, tx
either Wegner's or microscopic polyangitis; form of pauci-immune glomerulonephritis form of nephritic syndrome; LM: vasculitis w/ fibrinoid necrosis is diagnostic but rarely seen, focal necrotizing GN w/ crescents is commonly seen but not specific; FM: negative Ig/complement (hence pauci-immune), w/ fibrin in Bowman's space; EM: collapse of glom tuft, no immune deposits; also positive for ANCA on serology; tx w/ immunosuppresion (95% remit w/ freq relapses)
70
serologies (6)
ANCA positive in pauci-immune GN due to vasculitis; anti-dsDNA, ANA, hypocomplementemia positive in lupus; hypocomplementemia positive in post-infx; ASO positive in post-strep
71
consequences of tubulointerstitial disease (8)
reduced GFR (AKI or CKD), Fanconi syndrome, RTA, tubular (small molecular weight) proteinuria (<2 g/day), polyuria, isothenuria (Uosm = Posm b/c tubules can’t concentrate or dilute), hyperkalemia (no K secretion), salt wasting (no Na reabsorption)
72
ATN etiologies
ATN is most common cause of AKI; due to ischemia or toxins (drugs, heme pigements, contrast agents)
73
acute interstitial nephritis LM
inflammation (mostly T cells) in interstitium; can cause tubulitis; assoc w/ edema that separates tubules; spares glomeruli and vessels (altho can be affected in chronic interstitial nephritis)
74
thyroidization occurs in
chronic interstitial nephritis -> atrophic, dilated tubules filled w/ proteinacious material
75
AIN etiology (7)
drug reaction (allergic) is most common, infection (direct renal infx or systemic); immunologic (lupus, Sjogrens, sarcoidosis, allograft rejection); idiopathic (tubulointerstitial nephritis-uveitis syndrome)
76
allergic interstitial nephritis presentation
classic triad (rash, fever, peripheral eosinophilia) is rare, us. one or two of those sx w/ malaise, fatigue, arthralgias, flank pain; these sx occur 10-14 days after first drug exposure or sooner after repeat exposure and are not dose-dependent
77
serum and urine in allergic interstitial nephritis
serum: elevated BUN/creatinine, eospinophilia; urine: lots of WBCs and WBC casts, occasional RBCs, mild proteinuria (can see nephrotic range w/ NSAIDs b/c they cause minimal change disease), eosinophiluria (>1% of WBCs, seen best w/ Hansel’s, not sensitive or specific), NO BACTERIA
78
diff dx of pulm-renal syndrome (3)
Goodpasture's, SLE, ANCA positive small vessel vasculitis
79
diff dx of eosinphiluria (4)
AIN, RPGN, atheroemboli syndrome, transplant rejection
80
drugs that lead to AIN (12)
antibiotics (pencillin analogues esp methicilin, cephalosporins, sulfonamides, rifampin, quinolones), NSAIDs, salicylates, loop and thiazide diuretics, phenytoin, allopurinol, cimetidine, omeprazole
81
AIN LM, pathophys
LM: dense inflammatory infiltrate (mostly lymphocytes w/ some eosinophils), occasional granulomas (sparse and non-necrotic); most drug-induced AIN is cell mediated (lots of T cells, no immune deposits)
82
when do we see "SICK" kidneys, and what does SICK stand for?
drug-induced (us. analgesic) chronic interstitial nephritis; SICK is small, indented, calcified gross kidneys
83
common cause of chronic interstitial nephritis: what is it, demographics, disease development, assoc w/, similar disease
analgesic nephropathy due to combo meds (aspirin + pehnacetin + caffeine); most common in women w/ chronic pain; assoc w/ papillary necrosis (-> polyuria due to vasa recta damage) and chronic interstitial nephritis -> progressive kidney failure and hypertension; incr. incidence of transitional cell carcinoma of uroepithelium; Aristolochic acid nephropathy (Chinese herbal nephropathy) has similar features
84
what disease has papillary necrosis and chronic interstitial nephritis?
analgesic nephropathy, Chinese herbal nephropathy (aristolochic acid nephropathy)
85
acute pyelonephritis: us. caused by, settings (3), LM, consequences, viral causes (2)
us. caused by gram negative directly invading kidney via ascending route; commonly settings: UT obstruction, reflux, pregnancy, diabetes; LM: pus casts in renal tubules, microabscesses on surface or w/in parenchyma; us. don't impair renal fn unless associated w/ V depletion or sepsis; viral causes: BK polyoma, cytomegalovirus
86
vesicouteral reflux: types, predisposes to what
primary VUR is due to incompetent or inadequate closure of uterovesical junction during micturition (common in kids); secondary VUR is due to high bladder P that results in UVJ closure failure (aka urethral obstruction); VUR predisposes to pyelonephritis, esp. at renal poles due to more compound papillae
87
chronic pyelonephritis
due to VUR; renal poles more affected (more compound papillae); focal scars over blunted calyces at poles; can occur w/o infx just due to sterile urine reflux
88
multiple myeloma cast nephropathy LM, gross
tubules distended by homogenous, eosinophilic, fractured casts surrounded by multinucleated giant cells; Myeloma kidney is waxy and large w/ preserved cortex (one of few diseases w/ enlarged kidneys in CKD)
89
multiple myeloma can affect kidney how (7)
misc: dehydration, hypercalcemia, hyperuricemia (tumor lysis syndrome), infection; light chain mediated: cast nephropathy, light chain glomerulopathy, amyloidosis
90
MM light chain glomerulopathy LM/FM, sx (3)
LM: light chains deposit in mesangium -> nodular glomerulopathy that resembles diabetic nephropathy, poss chains depositing in tubular BM (PAS would show thickening of BM); FM: monoclonal light chains in glomeruli and poss tubular BM; sx: proteinuria (poss nephrotic range), microscopic hematuria, renal failure, less commonly Fanconi's
91
MM amyloidosis: involve what part of kidney (2), sx (2)
us involve glomeruli and blood vessels; pts present w/ heavy proteinuria and renal insufficiency
92
main histologic feature of chronic interstitial nephritis
interstitial fibrosis w/ tubular atrophy
93
normal urine
0-3 RBC/hpf, 0-2 WBC/hpf, few epithelial cells, 0-1 hyaline cast/lpf, up to 150 mg/day protein (+1 on dipstick if urine is concentrated)
94
diff dx of red-brown urine
if sediment red (RBCs) then hematuria; if supernatant red and dipstick neg, then beeturia, phanazopyridine, porphyria; if supernatant red and dipstick positive, then myoglobin or hemoglobin; if hemoglobin then plasma should be red, if myoglobin then plasma should be clear; brown urine can be due to acid in urine turning hemoglobin into brown methemoglobin
95
turbidity of urine diff dx
normal: contaminating mucus, precipitating urate/phosphate crystals (esp if refrigerated); abnormal: microorganisms, cells, casts, crystals, lipids; foamy: heavy proteinuria
96
microalbuminuria
albuminuria ( > 20 mcg/min or > 30 mg/day) w/o proteinuria ( < 150 mg/day); highly predictive of clinical nephropathy in DM and CV dis in T2DM and nondiabetics; need special assay to detect (RIA or ELISA)
97
urine epithelial cells
squamous (large, flat, small nucleus) due to urogenital contamination; tubular (1.5-3x bigger than WBC, round nucleus) due to ATN, exercise, GN
98
Maltese cross
if heavy proteinuria present, tubular epithelial cells can undergo fatty degeneration and form Maltese crosses under polarized light -> fatty casts seen in glom disease w/ heavy proteinuria (>3 g/day)
99
Casts (7 - what are they, describe them, due to)
hyaline casts (colorless cylinders), waxy and broad casts (high optical density, may have cracks, seen in advanced renal failure), granular casts (degen cells or aggregated serum proteins in many diseases) or muddy brown cigar casts (seen in ATN), epithelial cell casts (ATN, tubulointerstitial disease), RBC cases (GN), WBC casts (tubulointerstitial dis, pyelonephritis, acute GN), fatty casts (glom disease w/ heavy proteinuria aka nephrotic syndrome -> Maltese cross under polarized light)
100
conditions favoring casts (4)
acidic urine, concentrated urine, urine stasis, proteinuria
101
crystals (5 - what are they)
uric acid, calcium oxalate, struvite, calcium phosphate, cystine
102
uric acid crystal: description (3), pH, seen in (4)
yellow or reddish-brown; may cause cloudy pink urine on precipitation; pleomorphic, rosettes, needles, or diamond shaped; found in acidic urine; seen in gout, fever, cytotoxic therapy for lymphoma (marked increase in uric acid production), amorphous urate is common and not clinically significant
103
calcium oxalate crystals: description (2), pH, seen in (6)
envelopes (oXalate = X on envelope) or dumbbells, independent of pH; found in increased oxalate production (ethylene glycol ingestion, methoyxflurane anesthesia, glycerol infusion, primary hyperoxaluria) or absorption (chronic diarrhea w/ malabsorption), renal failure
104
struvite crystals: aka (2), description, pH, seen in (2)
aka magnesium ammonium phosphate aka triple phosphate; coffin-lids; alkaline urine; assoc w/ UTI w/ urease producing bacteria (Proteus mirabilis), assoc w/ staghorn calculi
105
calcium phosphate crystals: description (3), pH, seen in
pleomorphic, amorphous, stellate; seen in alkaline urine; seen commonly
106
cystine crystals: description, pH, seen in
hexagonal, us. independent of pH although less common at pH > 7.4; seen in cystinuria
107
crystals in acidic urine (3)
uric acid (acid urine only), calcium oxalate (all pHs), cystine (all pHs but less common above 7.4)
108
crystals in alkaline urine (4)
calcium phosphate, struvite, calcium oxalate (all pHs), cystine (all pHs but less common above 7.4)
109
coffin-lid crystals
struvite crystals
110
envelope crystals
calcium oxalate crystals
111
dumbbell crystals
calcium oxalate crystals
112
cloudy pink urine on precipitation
uric acid crystals
113
pleomorphic, rosettes, needles, or diamond shaped crystals
uric acid crystals
114
pleomorphic, amorphous, stellate crystals
calcium phosphate crystals
115
hexagonal crystals
cystine crystals
