GREEN - MOA

Question 1

Macrolides block the ___ ribosomal subunit and block transaction reaction.

Answer 1

50s

Question 2

___ binds to the 30s ribosomal subunit and prevent tRNA attachment

Answer 2

Tetracycline

Question 3

Absorption of tetracyclines are impaired by ___ and ___ because of chelation

Answer 3

mineral and antacid: calcium, magnesium, iron, dairy including milk

Question 4

What is difference between bactericidal vs bacteriostatic?

Answer 4

Bactericidal = kill bacteria
Bacteriostatic = prevent growth

Question 5

Penicillin mimics ____ (aa) and binds to ____ to inactivate the enzyme and causes autolysis.

Answer 5

1. alanine

2. transpeptidase

Question 6

Cephalosporins have ______ ring, but not ______ ring like penicillin.

Answer 6

1. B-lactam

2. thiazolidine

Question 7

True or False - bacteria has a 70s ribosome composed of a 50s and 30s subunits

Answer 7

TRUE!

Question 8

The binding sites inside a ribosome:
A. E-P-A
B. A-B-P
C. E-T-A
D. E-R-B

Answer 8

A. E=exit, P=peptidyl, A=aminoacyl

Question 9

Penicillin G blocks formation of new bacterial ______

Answer 9

cell walls - inhibits cross-linking of peptidoglycan

Question 10

A 62-year-old man comes to his primary care physician because of a penile discharge and a painful right scrotum that started 3 days before. The patient states he has experienced similar symptoms in the past when he was diagnosed with syphilis. When asked about sexual activity, the patient states that he recently started seeing a widowed woman and has been having unprotected sex with her. A nucleic acid amplification test (NAAT) confirms the presence of a gram negative intracellular organism. A few days later he calls the physician back concerned that his fingernails are turning blue. Which of the following best explains the mechanism of action of the antibiotic used?

A. DNA-dependent RNA polymerase
B. Blockage of tRNA-binding to the 30s ribosome complex
C. Inhibition of peptidoglycan synthesis
D. Nucleoside reverse transcriptase inhibition

Answer 10

B - Tetracycline antibiotics are widely known for their potential to discolor the skin, nails and teeth, classically with a blue-grey hue.

The patient in this question most likely has a genitourinary chlamydial infection caused by C. trachomatis. C. trachomatis is a gram negative obligate intracellular bacteria, often sexually transmitted

Question 11

MOA of all Cephalosporin is the inhibition of transpeptidase, thereby inhibiting the cross-linking of ____ found in bacteria’s ____

A. Peptidoglycan; nucleus
B. Collagen; cell membrane
C. Peptidoglycan; cell wall
D. tRNA; cytoplasm

Answer 11

C - peptidoglycan; cell wall