Gram -ve Cocci

Question 1

Characteristics

Answer 1

Gram -ve diplococci
Aerobics
Oxidase

Produce acid from sugar oxidation Non-fermentative Catalase +ve

Can grow both intracellularly and extracellularly.

not blood agar but chocolate 🍫

LOSnot LPS

Question 2

Culture M,G

Answer 2

Chocolate agar: Colonies are: very smooth, transparent, non-pigmented

1. Chocolate agar (enriched)
2. Thayer-Martin agar (selective)
3. Modified Thayer-Martin agar

Question 3

Virulence factors M G:

Answer 3

1. Anti-phagocytic polysaccharide capsule 2. Endotoxin (LOS) 3. Pili (fimbriae)
2. IgA protease
3. Factor H binding protein (FHBP)

[1] Pili: inhibits phagocytosis
[2] IgA protease
[3] Three outer membrane proteins:
Protein I (por protein)
Protein II (Opa Proteins)
Protein III (Rmp)

[4] Endotoxin (LOS)

Question 4

Disease M,G

Answer 4

[1] Meningitis
[2] Meningococcemia (septicemia): Water house- Friderichsen syndrome :(DIC), thrombocytopenia and adrenal Insufficiency,

شوفي الشيت 👀

Question 5

Treatment M,G

Answer 5

Analgesics: for pain
IV fluids: to avoid shock
Corticosteroids: to reduce intracranial pressure and fever
Antibiotics: Penicillin G ceftriaxone

Ceftriaxone
azithromycin or doxycycline هنا عشان معاها Cl. trachomatis

Ophthalmia neonatorum treatment: Ceftriaxone injection

Ophthalmia neonatorum prophylaxis:

• Silver nitrate 1% aqueous solution:

topical eye drop

• Erythromycin ointment