Gram Staining & AFB Flashcards
Primary Stain of Gram Staining
Crystal Violet (1 min)
Mordant for Gram Staining
Gram’s Iodine (1 min)
Decolorizer for Gram Staining
Acetone alcohol (10 seconds or less)
Counterstain for Gram Staining
Safranin (30 secs)
Primary Stain for AFB (Fluorescent Stain)
TB auramine-rhodamine T stain (25 mins)
- Auramine-O
- Auramine-O-Rhodamine-B
Decolorizer for AFB (Flourescent Stain)
0.5% acid alcohol (2 mins)
Counterstain for AFB (Fluorescent Stain)
potassium permanganate (4 mins)
Primary Stain for AFB (Kinyoun and Ziehl-Neelsen Stain)
Carbolfuchsin (5 mins)
Decolorizer for AFB (Kinyoun and Ziehl-Neelsen Stain)
acid alcohol
70% ethanol (Modified; Partial Acid-Fast)
Counterstain for AFB (Kinyoun and Ziehl-Neelsen Stain)
methylene blue (1 min)
- for modified; PAS, 30 seconds
Components of a PLASMA MEMBRANE
- Lipid bilayer
- Protein
- Carbohydrates
Types of Staining
- Simple (methylene blue, iodine)
- Differential (gram staining, AFB)
- Negative (India ink, nigrosin stain)
- Results of the bacteria appearing as light-colored bodies against the background
- for organisms who have CAPSULE
Negative Staining
Who created the Gram Staining
Hans Christian Gram (worked with Carl Friedlander)
In the cell wall of the gram-negative, what are found in the OUTER MEMBRANE?
- Porin
- Lipopolysaccharide/ Endotoxins
