Gram stain

Question 1

What is a gram stain used to differentiate?

Answer 1

Bacteria

Question 2

When preforming a gram stain, what is the proper order of reagents?

Answer 2

Crystal violet, Gram’s iodine, acetone-alcohol, safranin

Question 3

The destruction of all microorganisms and their endospores is referred to as:

Question 4

Straight-rod shaped bacteria, is referred to as:

Answer 4

Bacilli

Question 5

When interpreting a gram stain you should view bacteria on what lens?

Answer 5

100x (oil immersion)

Question 6

A quality control gram stain smear was prepared using an 18-hour-old culture of Escherichia coli (gram negative rod). On microscopic examination the gram stain smeared organism appeared blue, this means:

Question 7

An organism that gram stains purple and has a shape of round spheres would be:

Answer 7

Gram positive cocci

Question 8

An organism that gram stains pink and has a shape of long narrow rods would be:

Answer 8

Gram negative bacilli