Gram Positive Tests : What Do They Test For? Rational?

Question 1

catalase test tests for what?

Is it differential? If so for what?

Answer 1

enzyme catalase

Question 2

what occurs in order to get a positive result for a catalase test?

Answer 2

the break down of H2O2 into H2O and O2

O2 is used as the final electron acceptor

Question 3

What does a postive resault for a catalse test look like?

Answer 3

bubbles

Question 4

catalase differentiates between what?

what one is pos.? Neg.?

Answer 4

staphylococci (pos)

streptococci (neg)

Question 5

Coagulase test is used to differentiate what?

Answer 5

Differentiates Staphylococcus aureus from other a-hemolytic bacteria.

Question 6

what occurs in order to get a positive result for a coagulase test?

Answer 6

Coagulase is an enzyme that converts soluble fibrinogen to insoluble fibrin threads in the blood, to form clots (protects bacteria from host immune system)

Question 7

what does a positive coagulase test look like? what must it be?

Answer 7

there is a clot in the bottom of the tube

Staphylococcus aureus

Question 8

What is the NB Disc test used for?

What does NB stand for?

Answer 8

differentiating between Coagulase Negative Staphylococci

Novobiocin

Question 9

describe the sensitive result for the NB Disc test?

What bacteria are sensitive?

Answer 9

> /= 16mm

Staph A, Staph E, & Micro L

Question 10

describe the resistant result for the NB Disc test?

What bacteria is resistant?

Answer 10

< 12mm

Staphylococcus Saprophyticus

Question 11

what occurs in oder to get a-hemolysis (alpha) result on Hemolysis on Blood Agar indicate?

Answer 11

partial hemolysis, red blood cell hemoglobin is reduced to methemoglobin