GRAM POSITIVE COCCI Flashcards
It is a spherical, non-motile grape like cluster gram positive cocci
Staphylococcus
They are non-encapsulated and nonspore former gram positive cocci
Staphylococcus
Staphylococcus that cannot ferment glucose (Glucose non-fermenters)
Staphylococcus saprophyticus
“BUNCHES OF GRAPES”
Staphylococcus
It appears creamy, white, or rarely light gold in Mannitol Salt Agar
Staphylococcus aureus
It is a test to differentiate gram positive cocci (Streptococci and Staphylococci)
Catalase Test
Obligate anaerobe Staphylococcus
Staphylococcus saccharolyticus
Reaction to Catalase
3% H202
A gram positive cocci that is catalase positive and coagulase negative
Micrococcus luteus
The colony of Micrococcus appears in what color?
Distinct yellow pigmented colony
Habitat of Staphylococcus aureus
Anterior Nares
Give the 3 Clinically important species of Staphylococci
S. aureus
S. epidermidis
S. saprophyticus
A modified catalase contains what?
Mild Detergent
Mannitol Salt Agar colony appears in what color?
Yellow/Golden Yellow pigments
What is the Mode of Transmission of Staphylococcus aureus?
Traumatic introduction/entry
“Oil Paint appearance”
Staphylococcus aureus
“lemon yellow pigment”
Staphylococcus citreus
“porcelain white pigment”
Staphylococcus albus
“porcelain white pigment”
Staphylococcus albus
What is the color of the colony in K tellurite medium?
Jet Black Colonies
Staphylococcus specie that is human pathogen
Staphylococcus aureus
Staphylococcus species that is Animal pathogen
(DISH)
S. delphini
S. intermedius
S. schleiferi
S. hyicus
Staphylococcus that can be acquired in hospital infections
Staphylococcus epidermidis
Staphylococcus that can cause UTIs in young sexually active females
Staphylococcus saprophyticus
Staphylococcus aureus is NOSOCOMIAL. What do you mean by Nosocomial?
Hospitally acquired
It is a dangerous Staphylococcus specie
Staphylococcus saprophyticus
Staphylococcus Species that are coagulase positive
(DISH)
S. delphini
S. intermedius
S. schleiferi
S. hyicus
It produces beta hemolytic (complete hemolysis)
Staphylococcus
Beta hemolytic on Blood Agar Plate
Staphylococcus aureus
Sulfide Indole Motility (SIM) Test appears what in what color if motile?
Black
A gram positive cocci that is catalase positive and coagulase positive
Staphylococcus aureus
Singly, in pairs, & in cluster
Staphylococcus
Staphylococcus that is pus formers
Staphylococcus aureus
Coagulase is a _____________ factor
Clotting factor
Hyaluronidase is a ____________ factor
Spreading factor
What is an enzyme that breaks down fats & oil?
Lipase
Hemolysin that destroys the platelets and tissues
a hemolysin
Heat-stable exotoxins that cause diarrhea and vomiting (Food Poisoning)
Enterotoxins
Resistant to gastric acid and associated with food poisoning
Toxins A-E and G-I
It causes toxic shock syndrome
TSST-1 (Toxin F)
A virulence factors of Staphylococcus aureus that are superantigens
TSST-1 and B, C, G, and I
It causes sloughing off of the skin and is known to cause Scalded Skin Syndrome (SSS) or Ritter’s Disease
Exfoliative toxin (epidermolytic toxin)
It is also associated with bullous impetigo
Exfoliative toxin (epidermolytic toxin)
Kills polymorphonuclear leukocytes
Leukocidins (Panton-Valentine leukocidin)
It helps prevent phagocytosis
Leukocidins (Panton-Valentine leukocidin)
It is the ingestion of bacteria
Phagocytosis
Hemolysin that acts on the sphingomyelin of RBC membranes causing lysis
B hemolysin
Hemolysin that causes injury to cells and leukocytes but is less lethal
ō hemolysin
It can also break down sebaceous glands
Lipase
It blocks phagocytes
Protein A
Types of Hemolysin in Blood Agar: partial/narrow zone
Alpha
Types of Hemolysin in Blood Agar: complete
Beta
Types of Hemolysin in Blood Agar: non-hemolytic
Gamma
Types of Hemolysin in Blood Agar: wide/double zone
A prime
This bacteria that can be seen in blood agar have only one double zone hemolysin
Clostridium perfringens
Infection of the hair follicle
Folliculitis
REDNESS and this can be spread by direct contact and fomites which is highly contagious
Bullous impetigo
It is more likely to occur in renal failure patients
Staphylococcal Scalded Skin Syndrome (SSSS)
This syndrome is associated with super absorbent tampons
Toxic Shock Syndrome