Gram Positive Bacilli- Spore Formers

Question 1

Agent of anthrax

Answer 1

Bacillus anthracis

Question 2

Fried Rice poisoning

Answer 2

Bacillus Cereus

Question 3

Incubation period of diarrheal toxin. Heat stabe or not?

Answer 3

8-16 hours
Not heat stable

Question 4

Emetic toxin incubation period. Heat stabel or not?

Answer 4

15 hours incubation
Heat stable
Fried and boiled rice
Vomitting

Question 5

Bacillus cereus positive tests

Answer 5

Hemolysis
Lecithinase and motility
Gelatin hydrolysis

Question 6

Diagnostic precipitation test for B. Antracis

Answer 6

ASCOLI TEST

Question 7

Appearance of colony in B. Cereus

Answer 7

FROSTED GLASS

Question 8

Colony appearance in B. Anthracis

Answer 8

BAMBOO POLE colony

Question 9

Media used in B. anthracis

Answer 9

SBA- Medusa head/Comet tail/ Lion Head
GELATIN STAB- inverted fig tree
MHA w/ 10u Penicillin - STRING OF PEARLS
PLET MEDIUM- polymixin
Lysozyme
EDTA
Thallous acetate

Question 10

Also known as HAY BACILLUS

Answer 10

Bacillus subtilis

Question 11

Used in GUTHIE TEST and source of BACITRACIN

Answer 11

B. subtilis

Question 12

Associated with FLATSOUR SPOILAGE

Answer 12

B. stearothermophilus

Question 13

BOXCAR-SHAOED BACILLI

Answer 13

Clostridium perfringens

Question 14

Diseases associated with C.perfringens.

Answer 14

Gas gangrene- MYONECROSIS

Necrotizing fasciitis or enteritis necrotians- PIGBEL

Question 15

C.perfringes result in BAP

Answer 15

DOUBLE ZONE OF HEMOLYSIS

Question 16

Stormy fermentation in C.perfringens

Answer 16

LITMUS MILK

Question 17

Other name of C. botulinum

Answer 17

CANNED GOOD BACILLUS

Question 18

Tests positive in C.perfringens

Answer 18

EGG YOLK AGAR - lecithinase
NAEGLER TESTS
CAMP TEST - bow tie pattern
REVERSE CAMP- Arrow head

Question 19

Lipase positive/Demonstration of NEUROTOXIN
OVAL AND SUBTERMINAL PORES

Answer 19

C. Botulinum

Question 20

Diseases associated with C. Botulinum

Answer 20

Flaccid paralysis
Infant botulism

Question 21

Tack head/ Drumstick
Lollipop bacillus
Agent of tetanus

Answer 21

Clostridium tetani

Question 22

Associated with pseudomembranous colitis and antibiotic associated diarrhea

Answer 22

C. Difficile

Question 23

Swarming but slow growing

Answer 23

C.tetani

Question 24

Yellow colonies on CCFA

Answer 24

C. difficile

Question 25

Disease associated with C. tetani

Answer 25

TETANOSPASMIN- spastic paralysis TRISMUS- lock jaw DEVILS GRIN RISUS SARDONICUS

Question 26

Chartreuse flourescence Horse -stable/Barnyard Odor Demonstration of B toxin

Answer 26

C. Difficile

Question 27

Resemble Medusa head

Answer 27

C. Septicum