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Clinical Bacteriology > Gram Negative Cocci > Flashcards

Gram Negative Cocci Flashcards

1
Q

Bacteria that resembles gram negative cocci morphology

A

Moraxella catarrhalis

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2
Q

Most common of all Moraxella

A

Moraxella catarrhalis

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3
Q

Normal flora of the Upper Respiratory Tract (Oropharynx) that is OPPORTUNISTIC

A

Moraxella catarrhalis

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4
Q

3rd most common cause of Otitis Media and Sinusitis

A

Moraxella catarrhalis

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5
Q

Old name of Moraxella catarrhalis

A

Branhamella catarrhalis

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6
Q

Disease caused by Moraxella catarrhalis among children

A

Otitis media
Sinusitis

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7
Q

Biochemical Test for Moraxella catarrhalis

A

(+) Catalase
(+) Oxidase

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8
Q

Culture used in Moraxella catarrhalis

A

Chocolate agar plate

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9
Q

Appearance of Moraxella catarrhalis in chocolate agar plate

A

Pink to brown colony “Hockey puck” appearance colonies remain intact when pushed

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10
Q

What laboratory test is Moraxella catarrhalis positive

A

DNase test
Butyrate Esterase test

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11
Q

Laboratory test for Moraxella catarrhalis

A

CHO utilization
DNase test
Butyrate Esterase test

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12
Q

Only Neisseriaceae that can lyse DNase

A

Moraxella catarrhalis

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13
Q

Color of positive in Butyrate esterase

A

Blue

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14
Q

Specimen of choice for Butyrate esterase

A

Eye and Ear culture

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15
Q

What are the non pathogens Neisseria

A

Neisseria cinerea
Neisseria elongata
Neisseria flavescens
Neisseria lactamica
Neisseria mucosa
Neisseria polysaccharea
Neisseria sicca
Neisseria subflava

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16
Q

Non pathogenic Neisseria, colony is similar to Neisseria gonorrhoeae on Chocolate Agar Plate and differentiated by Colistin

A

Neisseria cinerea

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17
Q

Non pathogenic Neisseria with yellow pigment, assachrolytic and can grown on Blood Agar Plate and Chocolate Agar Plate at 22C

A

Neisseria flavescens

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18
Q

Non pathogenic Neisseria that is common in nasopharynx of infants and children and Meningococcal carrier surveys - common in<2 years old and rare in children

A

Neisseria lactamica

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19
Q

Non pathogenic Neisseria that is large, adherent to agar and very mucoid. It is also isolated in the airways of dolphins, nasopharynx of children and young adults

A

Neisseria mucosa

20
Q

Non pathogenic Neisseria that is Dry wrinkled, adherent and breadcrumbs like colonies on Blood Agar Plate

A

Neisseria sicca

21
Q

Non pathogenic Neisseria that is rod shape gram-cocci which is weakly positive to positive catalase test

A

Neisseria elongata

22
Q

Pathogenic Neisseria that is rod shaped gram-cocci, normal flora for DOGS, human infection after dog bite. (+) for catalase (-) acid from COH and weak (+) for Phenylalanine Deaminase Test

A

Neisseria weaveri

23
Q

Biochemical Test for Neisseria gonorrhoeae

A

Carbohydrate utilization test
Quadferm+
Oxidase test
Superoxol test
DNase test
Beta lactamase test
Chromogenic test

24
Q

Composition of Carbohydrate utilization test

A

Cystine Trypticase Agar + 1% carbohydrate

25
Q

Positive result in carbohydrate utilization test

A

Yellow color after incubation

26
Q

Composition of QUADferm

A

Glucose
Maltose
Lactose
Sucrose

27
Q

Fermented by Neisseria gonorrhoeae

A

Glucose

28
Q

Fermented by Neisseria meningitidis and not fermented Neisseria gonorrhoeae

A

Maltose

29
Q

Used to differentiate Neisseria gonorrhoeae and Neisseria meningitis

A

QUADferm - Maltose

30
Q

Positive result in QUADferm test

A

Red to yellow color

31
Q

Test that detects cytochrome oxidase enzyme

A

Oxidase test

32
Q

Positive result in Oxidase test

A

Dark purple within 10 seconds

33
Q

Modification test

A

Superoxol test

34
Q

Reagent in Superoxol test

A

30% H2O2 (Hydrogen peroxide)

35
Q

Positive result in Superoxol test

A

Vigorous bubbling

36
Q

Test used to differentiate Moraxella catarrhalis (+) from Neisseria (-)

A

DNase test

37
Q

Positive result in DNase test

A

Clear halo around the colony

38
Q

Positive result in Beta lactamase test

A

Deep pink or red within 10 minutes

39
Q

Causes Meningitis

A

Neisseria meningitidis

40
Q

Collection of Neisseria meningitis

A

Pharyngeal swab

41
Q

Pathogen

A

Commensal and True pathogen of the upper respiratory tract

42
Q

Causative agent for

A

Meningococcemia
Spotted fever

43
Q

Mode of transmission

A

Close contact (respiratory droplets)

44
Q

Culture

A

Blood agar plate
Chocolate agar plate
Thayer martin agar

45
Q

Major serogroups

A

A, B, C, Y & W135

Clinical Bacteriology (2 decks)

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