Gram + cocci to gram neg aerobic cocci Flashcards
virulence factor that supress/inhibits phagocytosis exotoxins that kills wbc
Leucocidin
Another name for Leucocidin
Panton Valentine
The golden yellow pigment in Staphylococcus aureus is due to
Staphyloxanthin
Golden grape like clusters also called buttery or old paint appearance
Staphylococcus aureus
Large smooth, white or pigmented creamy yellow colonies
BAP (Blood agar plate)
Meaning of CNA
Colistin Nalidixic Acid
Can be used as base media for BAP
Columbia CNA
Responsible for boils. Initiates skin infection
Lipase
Free. Marker of virulence. converte fibrinogen to fibrin
Coagulase Clumping factor A
Also called as Duran-Raynal Factor. A spreading factor
Hyaluronidase
Dissolves fibrin clot and also called as fibrinolycine
Staphylokinase
Virulence factor resistance to penicillin
Beta Lactamase
Virulence factor that has toxic effects on neutrophils
Urease
Virulence factor in food poisoning
Enterotoxin A-F (Neurotoxin)
Virulence factor that also kills wbc
Protein A (cell wall)
Transmission of fomites
Aerosol
Transmission of Surgical wounds
Abrasion
Number 1 cause of Musculoskeletal
Staphylococcus aureus
Three types of intoxication
Food poisoning, TSST-1 and SSSS
Gastroenteritis or food poisoning occurs how many hours after ingesting toxin?
1-8 hours
Type of enterotoxins commonly associated with food poisoning
Types A and D enterotoxins
Types of enterotoxin that is associated with Toxic shock syndrome
Enterotoxin F and enterotoxin C
Tampons are associated with
Toxic shock syndrome (TSS)
Facilitates intracellular adhesion on skin limited to stratum. Occurs in babies or newborns
Staphylococcal Scalded Skin Syndrome (SSSS)
Also called as Pemphigus neonatorum or Ritter’s disease or Localized bullous impetigo
Staphylococcal Scalded Skin Syndrome (SSSS)
Gram stain must be cultured within
24-36 hours (maximum 2 days)
Test for bound/clumping factor and a screening test
Slide test
test for Free coagulase and a confirmatory test
Tube test
Anticoagulant of tube test in coagulase test
EDTA
Meaning of CONS
Coagulase Negative Staphylococci
Reagent that gives better clotting effects and free from inhibitor.
Rabbit EDTA plasma
How many ml of Rabbit EDTA Plasma?
0.5-1ml
Reagent that gives false positive in coagulase test
Citrate
ml of NaCl Staphylococcus aureus needs to grow
7.5 to 10%
Medium of Mannitol salt agar
7.5% NaCl
Test that is high in salt content
Mannitol salt agar
Indicator of Mannitol salt agar
Red
Differentiate S. aureus from S. intermedius
Voges-Proskauer
Result of Voges-Proskauer
Pink/Burgundy/Maroon
How many units of Bacitracin in S. aureus and S. pyogenes
0.04 units
Other Dnase positive
S. aureus, Moraxella and Serratia
Performed on BAP
Furazolidone
Result of clearing of agar
DNase test
DNA + Methyl green = Clear zone
Staphylococcus aureus
DNA + Toluidine blue = color of the zone?
pink zone
Color of positive beta lactamase production
pink to red
spp of staph that is gram positive in tetrads. Catalase positive and coagulase positive
Micrococcus
Meaning mRSA
Methicillin resistant Staphylococcus aureus
Culture of staph and strep
phenyl, ethyl, alcohol, agar (PEA)
Spp of staph not associated in human infection and used in animal feeds, dairy and cosmetics
Micrococcus
spp of staphy associated with marine environment
Planococcus
spp of staph, cuboidal packages and can be cultivated from stomach contents
Sarcinae
Another term for Rothia mucilaginosa
Stomatococcus mucilanginosus
spp of staph isolated from the mucous membrane of the cheek and gingiva. emerging pathogen in immunocompromised patients
Rothia mucilaginosa
spp of staph Frequent cause of endocarditis and may cause catheterilated bacteremia
Staphylococcus lugdunensis
Ferments mannitol and slide coagulase positive
Staphylococcus lugdunensis
spp of staph positive in tube coagulase
Staphylococcus haemolyticus
spp of staph responsible for various nosocomial infections including bacteremia, brain abscess, and infection of intravenous pacemakers
Staphylococcus schleiferi
