Gram

Question 1

Which stains can be used in a bug panel to target gram positive and negative bacteria?

Answer 1

Gram stain
Gram Twort
Brown-Hopps (B&H)

Question 2

Which stains can be used in a bug panel to target acid fast bacteria?

Answer 2

ZN (for tuberculosis)
FITE (for leprosy)

Question 3

Which stains can be used in a bug panel to target H. pylori and spirochetes?

Answer 3

Giemsa
Steiner
Warthin-Starry

Question 4

Which stains can be used in a bug panel to target fungi?

Answer 4

Grocott methenamine silver (GMS)
Chromic Acid Schiff

Question 5

What is the purpose of using differential staining for bugs?

Answer 5

To divide all the bacteria into 2 groups based on the differing compositions of their cell walls using 2 stains

Question 6

What would a pathologist see in an H&E stain that would prompt them to order a bug stain?

Answer 6

Acute inflammation with neutrophils
Necrosis, abscess, granulomas
Increase in macrophages and lymphocytes

Question 7

What is the classification of the gram stain?

Answer 7

Microorganism stain

Question 8

What is the target for the gram stain?

Answer 8

Gram positive and negative microorganisms

Question 9

What is the staining principle of the Gram stain?

Answer 9

Differential staining