GPCOC:CLEAR:LARGE:Branching:Enteric GNROD:Antibitocs:Non Enteric:Spirochetes:MISC:Rickettsis:Mycology(fungus):Molds: Flashcards
Viruses
-need a host cell to replicate
-intracellular
-DNA/RNA(protein synthesis)
-contagious(flu/cold)
-can be fatal
-protein coat=CAPSID(has DNA/RNA never both)
-envelope=FRAGILE; virus is easier to inactivate host(easiest to kill)
non-envelope=TOUGHER; virus hard to inactive
-some can cause birth defects(TERATOGENIC)
-can be destroyed by heat/autoclave
Replication
I. ATTACHMENT(cell specific; FIV( cat-antibody detection); HIV(humans) but can affect all)
II.PENETRATION
III.UNCOATING(releasing of nucleic acid)
IV.REPLICATION(host cell makes more copies of strand of DNA or RNA)
V.VIRAL ASSEMBLY(put into new mature viruses)
VI.VIRAL RELEASE(replication uncontrallably)
Bacteria vs. Viruse
-Bacteria can grow on artificial media
VIRUSES: need living host cell; wont grow on media
-Bacteria have DNA/RNA
VIRUSES: only havr one or the other
-Bacteria respond to antibitocs**
VIRUSES do not**
Diagnosis of Vira Infection
I. Electron Microscope II. Histopathology III. Living Cell Culture IV. PCR(polymerase chain reaction: detects viral DNA/RNA) V.Immunoassay(serology); most common
D
N
A
Families
I. Parvoviridae
II. Herpesviridae
III. Adenoviridae
-VACCINE AVAILABLE(Cat/Dog)
Parvoviridae
I. Canine Parvo
II. Feline Panleukopenia(feline distemper)
-D/V
-VACCINE avvailable
Herpesviridae
I. Feline Herpies(FELINE RHINOTRACHEITIS)
II. Canine Herpies**(NOT TESTED ON)
III. Herpesvirus Simiae
-VACCINE available
Adenoviridae
I. ICH(INFECTIOUS CANINE HEPATITIS);TYPE I
-infected urine/feces
-too many side effects
II. DA II- canine Adenoavirus ; TPYE II; Tracheobronchitis
-kennal cough
-bordetella
R
N
A
Families
I.Rhabdoviridae II.Retroviridae III.Paramyoxoviridae IV.Coronaviridae V.Caliciviridae
Rhabdoviridae
I.RABIES -zoonosis -VACCINE available II.FIV(Feline Immunodeficency) -feline aids -fatal -vaccine available III.FeLV(Felina Leukemia virus) -fatal -VACCINE available -mutates -causes Lymphoma**
Paramyxoviridae
I.Canine Distemper -serious in dogs -common in puppies -VACCINE available -through inhilation II. Canine Parafluenza -contributes to kennal cough -VACCINE available
Coronaviridae
I. Corona Virus -similar to parvo -D/V -VACCINE available II. FIP(Feline Infectious Peritonitis) -anorexia -fatal to cats -VACCINE available; not effective and expensive
Caliciviridae
- Feline Calici Virus
- upper respiratory infection
- VACCINE available
Togaviridae
-Horse Virus
- ***EQUINE ENCEPHALOMYELITIS
- transmitted by mosquito
- similar to west nile virus
- EEE
- WEE
- VEE
- horses are immunized
- ***Rhabdoviridae
- oral lesions
- NO VACCINE
- Retroviridae
- EIA
- coggins test
- NO VACCINE
Flavivirdae
- Horse Virus
- West Nile Virus
- similar to equine encephalomyelitis
- vector: mosquito
- not fatal to dogs/cats
- affects horse/humans
Herpesviridae
-Cattle Virus
-***Infectious Bovine Rhinotracheitis(IBR)
Retroviridae
-Cattle Virus
- Bovine Viral Leukosis(BLV)
- NO VACCINE
Swine Viral Infection
- swine influenza
- pseudorabies
Sheep/Goats Viral Infection
- ORF: Contagious ecthyma
- zoonotic
- VACCINE available, but cn get ORF from it
MOLD
Divisions:
- I. Sapromyte
- II. Dermatophyte
-spore forming=CONIDIA**
-ID based on colony morphology both colony/plate
-always present in air
-***SPORANGOSPORES-all over
-sac like structure
-formed by tubular filaments called: HYPAE
Septate hyphae=division
Non-septate hyphae=no divison
-Mycelium:tangled mass of hyphae
-multicellular
Saprophyte
-MOLD
- common in environment
- free living
Dermatophyte
-MOLD
- causes skin infections: superficial infection
- RINGWORM-dermatophytosis or dermatomycosis
- living on dead materal
- DM or DTM diagnosing
- turns red if positive
- growth is light in color
- lesions
- ZOONOTIC
- hard to disenfect
- cats occurs on face and ears(PERSIAN CATS)
Mycotoxins
-bi product of mold
- grow on graind/things stored
- toxic ingested=MYCOTOXIXOSIS
- Diagnosing:
- get specimen(tissue)
- direct exam
- place specimen on fungal media(SAB)
- examin growth
- stained with LPCB(lactoohenal cotton blue)
- tease prep(SCOTCH TAPE PREP)
Conidospores
- Macroconidia
- Microconidia
formed at the free end of the hyphae
Microconidia
- small spore
- BLUE CANOES
- can be in clusters or single pairs
Microsporum canis
BLUE CANOES
-MOLD
-#1 cause in ringworm in dogs/cats
Microsporum gypsem
-MOLD
- found in soil(rual dogs common)
- lesions on front paws/nose
Tricophyton mentagrophyte
- species important in k9/cats
- 2nd common cause of ringworm
Diagnosing Ringworm
- Woods UV lamp
- pluck green glowing hairs( not all lesions glow)
- red=positive; light color means growth
- DTM testing
- Clear scotch tape
Aspergillus
-opportunistic mold
-common in the environment
-inhaled=aspergillosis
I.CANINE:
-rhinitis
-chronic nasal discharge
-common in farm dogs
-look like green velvet
II.BIRDS
-zoo penguins
-respiratory infection
-inhalation of spores
Dimorphics
-opportunistic mold
- outside mold
- mold(soil) @ room temp-yeast once enter tissue at body temp
- when inhaled cause body infections from soil
- respiratoy, but can get worse
Blastomyces
-opportunistic mold
- present mold in soil
- from mississippi river
- inhaled spores cause respiratory infection
- if systemically spread to lymph; to testicles in male dogs
Histoplasma
-opportunistic mold
- along mississippi river(river valleys)
- ppl test positive for it
- common enriched in bird/bat feces
- respiratory infection
- diarrhea
Coccidioides
-opportunistic mold
- VALLEY FEVER in humans
- causes fever
- SW US most common found
- causes respiratory infection
- cause CNS
Sportrichosis
-opportunistic mold
- northern US
- pus infections
- ROSE PICKERS DIEASE
Mycology
I. yeast
II. mold
III. dimorphics
- eukeryotic
- study of fungus
Yeast
- unnicellular
- larger than bacteria
- BUDDING is form of reproduction
- gram stain positive
- NO ANTIBIOTICS
- facutative anaerobes
DIAGNOSISNG:
I. grab specimen
II. direct ear cytology(purple peanut men)
III.SAB media
IV. examination of growth
V. biochemical test only if need to know genus species
Candida albicans
-yeast
- NF
- oral cavities of humans
- opportunistic pathogen
- THRUSH in humans=oral yeast
- immunocompromised
- vaginitis in woman
- IMPORTANT in birds
- THRUSH or SOUR CROP
- esophageal puch is affected
- causes bovine mastitis***
Cryptococcus neoforman
-yeast
- in pigeon poop
- pigeons show now sign of the yeast
- CAPSULEs
- common in environment
- oportunistic
- through inhilation
- form granulomas(head/mouth)
- respiratory infection
- DOGS will have CNS(meningitis)
DIAGNOSING:
I.india ink
II. capsules show well
III. halo or clear ring around sphere
Malassezia pachydermatis(BAD ASS)
-yeast
-NF
-oily skin/ears
-leasing cause of OTITIS in dogs
-dermatitis
DIAGNOSING:
I. swab ear
II. purple budding
Rickettsial Organisms
- Rickettsia
- Anaplasma
- Coxiella
- Ehrlichia
- Bartonella
- Neorickettsia
- intracellular
- wont grown on blood agar
- vectors important for transmission
Rickettsia rickettsii
-rickettsial
- RMSF=rocky mountain spotted fever(in ppl)
- TICK FEVER in dogs
- vector=tick
- INFLAMMATION OF BLOOD VESSELS=vasculitis
- hemorrhage due to thrombocytopenia
- Serology for diagnosing
Ehlichia canis
-rickettsial
- -suseptable to german shepards
- canine ehrlichiosis-tropical canine(vietnam)
- **Ehrlichia canis-canine monocytoic ehrlichiosis(CME)
- vector-tick
- lives in WBC
- ** thrombocytopenia and bleeding tendencies is most common
- serology diagnosing for antibodies
- **morule=intracytoplasmic inclusons of organisms in WBC
Ehrlichia risticii
-rickettsial
- POTOMIC HORSE FEVER(discovered in maryland)
- now NEORICKETTSIA risticii
- east
- most common vestor is fly
- mild colic
Anaplasma marginale
-rickettsial
- affect RBCs
- in RUMINANTS
- vector-tick
- most common in tick-borne disease in cattle
- transmission by blood contaminated instruments
Bovine Anaplasmosis
- lives in RBCs
- anemia
- lyces of RBC
Mycoplasmas
- lack cell wall
- free living cell
- fried egg colony
- candle jar
Mycoplasma bovis
-cause mastitis
Haemoplasmas
-formal genus -Haemobartonella
- Mycoplasma haemofelis
- Mycoplasma haemocanis(not tested on)
-blood-microplasmas
Mycoplasma haemofelis
-haemobartonellosis
- FIA(feline infectious anemia
- lives on RBC
- PCR/Serology
Bartonella henselae
-rickettsial
- CDC(cat scratch disease)
- vector=flea
- flirt firt=flea poop how humans get diease
- immunocompromised ppl
Mycobacterium
I. inhalation=pulmonary TB
II. ingestion=alimentary TB
-miscellaneous organism
- rod shaped
- lack cell wall
- dont gran stain
- ACID FAST=positive(pink)
- resistant to disinfectants
- ANTIBIOTIC resistant
- granulomas/tuberculosis lesions
- before pasterization of milk