GPC Flashcards
What is the proper incubation conditions of SA and colony morphology?
BA at 35C, gold and B-hemolytic
What is the proper incubation conditions of SS and colony morphology?
BA at 35C, white and non hemolytic
What is the proper incubation conditions of SE and colony morphology?
BA at 35C, white and non hemolytic
How to distinguish between Staph and streptococci
Staph is catalase positive
What is the proper incubation conditions of grpA strep and colony morphology?
BA at 35C anaerobic, 85% B-hemolytic, white
What is the proper incubation conditions of grub strep and colony morphology?
BA at 35C anaerobic, B-hemolytic, creamy and orange
What is the proper incubation conditions of pneumococcus and colony morphology?
BA candle jar. med white creamy with a-hemolysis and pneumolysin
What is the proper incubation conditions of viridans and colony morphology?
BA candle jar, tiny and white and on-hemolytic
What is the proper incubation conditions of enterococcus and colony morphology?
Ba candle jar, grayish and non-hemolytic
What are the confirmatory tests for SA?
coagulase-positive
What are the confirmatory tests for SS?
novobiocin-resistant
What are the confirmatory tests for SE?
novobiocin sensitive
What are the confirmatory tests for grpA strep?
bacitracin sensitive and latex agglutination
What are the confirmatory tests for grpB strep?
bacitracin resistant and hippurate hydrolysis
What are the confirmatory tests for pneumococcus?
optochin sensitive, bile soluble
What are the confirmatory tests for viridans
optochin resistant
What are the confirmatory tests for enterococcus?
6.5 NaCl growth, bile sculpin growth
Normal and pathogen: SA
normal: nose
pathogen: sputum
Normal and pathogen: SS
no normal
pathogen: urine
Normal and pathogen: SE
normal: skin
pathogen: wound and biofilm
Normal and pathogen: grpA strep
normal: URT
pathogen: throat swab, lesions
how to rule out grpA strep?
plate on BA and incubate anaerobically
Normal and pathogen: grpB strep
normal:none
pathogen: memningitis/CSF
Normal and pathogen: pneumococcus
normal: nasopharynx
pathogen: sputum
Normal and pathogen: viridans
normal: mouth
pathogen: blood, wound
Normal and pathogen: enterococcus
normal: gut
pathogen: urine, wound