GNDC

Question 1

Neisseria gonorrhoeae 
Colony appearance (3)

Answer 1

Small
Mucoid
Comes off plate as a whole colony, but sticky

Question 2

N. gonorrhoeae

Gram Stain

Answer 2

GNDC

Extracellular or Intracellular (in Segs.)

Question 3

GNDC colony growth conditions (3)

Answer 3

Grow in increased CO2 (3-5%)
If candle jar, use only white unscented candles
Incubate for 72 hours before calling it negative

Question 4

Moraxella catarrhalis

Colony appearance

Answer 4

Large

Can be pushed accross media like a hockey puck

Question 5

Neisseria meningitidis

Colony appearance

Answer 5

Medium

Moist

Question 6

Will GNDC grow on SBA?

Answer 6

Yes, but not as well as on chocolate. Must have Chocolate for N. gonorrhoeae

Question 7

What media do we use in order to decrease NF and obtain pure GNDC? (3)

Answer 7

Thayer-Martin
Modified Thayer-Martin
Martin-Lewis

Question 8

Name the three transport media for GNDC

Answer 8

JEMBEC
Transgrow
Special media with specialized bags to keep in CO2

Question 9

Name 5 different types of media that GNDC can grow on

Answer 9

5% SBA
Chocolate
Special Selective Media for (N. Gonorrhoeae)
Transport Media
NYC agar (lysed horse RBCs)
Enriched Broths (ex: Thio)

Question 10

What type of agar is TM and what does it contain?

Answer 10

It’s an enriched chocolate agar that contains:

• Colistin to inhibit G-
• Vancomycin to inhibit G+
• Nystatin to inhibit yeast