Glycolysis WTF DOES THIS ENZYME DO?!

Question 2

Hexokinase

Answer 2

Glucose –> Glucose-6-Phosphate

Requires ATP & Mg²⁺

Question 3

Phosphohexose-Isomerase

Answer 3

Glucose-6-Phosphate –> Fructose-6-Phosphate

Requires Mg²⁺

O attaches to C2 instead of C1

Pyranose –> Furanose

Question 4

Phosphofructokinase-1 (PFK-1)

Answer 4

Fructose-6-Phosphate –> Fructose-1,6-Bisphosphate

Requires ATP & Mg²⁺

Question 5

Adolase

Answer 5

Fructose-1,6-Bisphosphate –> Dihydroacetone Phosphate + Glyceraldehyde-3-Phosphate

Cleavage rxn.

Question 6

Triose Phosphate Isomerase

Answer 6

Dihydroxyacetone Phosphate –> Glyceraldehyde-3-Phosphate

G3P needed to move on!

Question 7

Glyceraldehyde-3-Phosphate Dehydrogenase

Answer 7

Glyceraldehyde-3-Phosphate + Inorganic Phosphate –> 1,3-Bisphoglycerate

Reduces NAD⁺ to NADH + H⁺

Question 8

Phosphoglyceratekinase

Answer 8

1,3-Bisphoglycerate –> 3-Phosphoglycerate

Requires Mg²⁺

Question 9

Phosphoglyceratemutase

Answer 9

3-Phosphoglycerate –> 2-Phosphoglycerate

Just changes Phosphate group position from C3 to C2

Question 10

Enolase

Answer 10

2-Phosphoglycerate –> Phosphoenolpyruvate

Removes H₂O, creates double bond

Question 11

Pyruvatekinase

Answer 11

Phosphoenolpyruvate –> Pyruvate

Requires Mg²⁺ & K⁺

Makes ATP