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Med > Glycolysis > Flashcards

Glycolysis Flashcards

1
Q

Glucose>

A

glucose 6 phosphate
enz: hexokinase
atp>adp

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2
Q

glucose 6 phosphate>

A

fructose 6 phosphate

enz: phosphoglucose isomerase

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3
Q

fructose 6 phosphate>

A

fructose 1,6 bisphosphate
enz: phosphofructokinase 1
atp>adp

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4
Q

F 1,6 BisP>

A

dihydroxyacetone phosphate AND glyceraldehyde 3 phosphate

enz: aldolase

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5
Q

dihydroxyacetone phosphate>

A

glyceraldehyde 3 phosphate
enz: triose phosphate isomerase
(2x GAD3P produced in total)

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6
Q

G3P>

A

1,3 bisphosphoglycerate
enz: glyceraldehyde 3 phosphate dehydrogenase
nad+ + pi >nadh + h+ (x2)

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7
Q

1,3 bisphosphoglycerate>

A

3 phosphogylcerate
enz: phosphoglycerate kinase
adp>atp (x2)

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8
Q

3 phosphogylcerate>

A

2 phosphogylcerate

enz: phosphoglycerate mutase

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9
Q

2 phosphoglycerate>

A

phosphoenolpyruvate
enz: enolase
>H20 (x2)

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10
Q

phosphoenolpyruvate>

A

pyruvate
enz: pyruvate kinase
adp>atp (x2)

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11
Q

net reaction of glycolysis

A

glucose + 2NAD+ + 2Pi + 2ADP > 2pyruvate + 2NADH + 2H+ + 2H2O + 2ATP

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12
Q

occurs where?

A

cytoplasm of cell

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13
Q

in aerobic or anaerobic resp?

A

glycolysis occurs in both aerobic and anaerobic respiration

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14
Q

where does the NADH product go?

A

oxidative phosphorylation

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15
Q

where does pyruvate go?

A

matrix (mitochondria) for link reaction (produces acetyl CoA)

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16
Q

preparatory and pay off phase?

A

prep phase= consume energy to convert glucose into GAD3P.
pay off phase is from GAD3P to pyruvate. Net gain of energy rich molecules ATP and NADH. Pay off phase occurs twice per glucose molecule, yielding 2ATP and 4NADH.

17
Q

what is the rate limiting step?

A

step 1 - glucose availability

step 3 - PFK-1 activation/inhibition

18
Q

positive regulators of glycolysis?

A

AMP, insulin

allosteric activation of PFK-1

19
Q

negative regulators of glycolysis?

A
  1. H+ (acidic enviro) - PFK-1 is ph dependant
  2. ATP 3. citrate - allosteric inhibition (AMP opposes allosteric inhibition)
  3. glucagon

Med (2 decks)

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