Glycolysis

Question 0

Which step is most regulated in the glycolytic pathway?

Answer 0

The conversion of fructose-6-phosphate to fructose 1,6 bisphosphate by phosphofructokinase.

Question 1

Which enzyme catalyses the conversion of glucose-1-phosphate to glucose-6-phosphate?

Answer 1

Phosphoglucose isomerase

Question 2

What is an allosteric inhibitor of phosphofructokinase?

Answer 2

ATP and citrate.

Question 3

Where does the phosphate to convert glyceraldehyde-3-phosphate to glyceraldehyde 1,3 bisphosphate come from?

Answer 3

Phosphoric acid

Question 4

What is an allosteric activator of phosphofructokinase?

Answer 4

AMP

Question 5

How is glyceraldehyde-3-P converted to phosphoenol Pyruvate?

Answer 5

Phosphorylation from phosphoric acid, dehydrogenation producing NADH and removal of one high energy phosphate to form ATP, then isomerisation to produce glycerate-2-phosphate and finally a dehydration reaction catalysed by aldolase.

Question 6

Draw out the conversion of glucose to fructose-1,6-bisphosphate

Answer 6

Glucose -> glucose-6-P -> fructose-6-P -> fructose-1,6-BP -> glyceraldehyde-3-phosphate (and dihydroxyacetone)