Glycolysis

Question 0

GLUT 2

Answer 0

liver, pancreas, low affinity (Kd = 15-20 mM)

Question 1

GLUT1,3

Answer 1

Ubiquitous, most cells, high affinity, Kd=1 mM

Question 2

GLUT 4

Answer 2

insulin induced, medium affinity (Kd = 5 mM), come to the membrane after a high carb meal

Question 3

SGLT

Answer 3

Another GLUT transporter - intestinal, kidney, for secondary active transport, symport, intestinal and kidney epithelial cells

Question 4

Glucose overview

Answer 4

Glucose goes to pyruvate (generates 2 ATP per glucose)
-kicks out NADH which is used and NAD+ is regenerated with the lactate dehydrogenase reaction (pyruvate to lactate, anaerobic conditions)

Question 5

GLUT 5

Answer 5

fructose transporter

Question 6

GLUT transporters. . .

Answer 6

act in response to concentration gradients

Question 7

Glucose can move against conc gradient bc. . .

Answer 7

passive transport of glucose is coupled with active transport of sodium ions

Question 8

Kd

Answer 8

ratio of bound versus unbound glucose to the transporter - if Kd is low, affinity for glucose is high

Question 9

Tumor cells

Answer 9

• often have high rates of glucose uptake and usage
• using radioactive glucose molecule to inject patients and visualize the cancer/path of glucose – detected with PET scanner

Question 10

NADH

Answer 10

generates 2.5 ATP

Question 11

FAD(2H)

Answer 11

Generates 1.5 ATP

Question 12

Complete oxidation of one Acetyl CoA

Answer 12

can generate 10 ATP

Question 13

Liver can uptake. . .

Answer 13

lactate to use in order to create glucose

Question 14

High Km

Answer 14

low activity

Question 15

Low Km

Answer 15

High activity