glycolysis Flashcards

1
Q

what has glycolysis got to do with carbohydrate metabolism?

A

It is the central pathway for carbohydrate metabolism as all sugars can be converted to glucose.

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2
Q

Is glycolysis an anaerobic or araeobic process?

A

An anaerobic process.

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3
Q

what organisms use glycolysis?

A

Mammals, bacteria and plants.

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4
Q

Where does glycolysis occur?

A

The cytosol in mammalian cells

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5
Q

Explain what happens briefly during glycolysis.

A

Glucose (C6) is broken down into 2 molecules of pyruvate (C3) and results in a net production if 2 ATP molecules.

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6
Q

How many reactions take place in glycolysis?

A

Ten

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7
Q

Explain phase 1 of glycolysis briefly.

A

Converts glucose to 2 glyceraldehyde 3-phosphate molecules. It is the energy requiring phase, catabolic.

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8
Q

Explain phase 2 of glycolysis briefly.

A

2 pyruvate molecules are generated. It is the energy yielding phase, anabolic

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9
Q

Products of glycolysis.

A

4 ATP, 2 pyruvate, 2 NADH, 2 H2O

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10
Q

What is the first irreversible reaction called & explain it.

A

It is called hexokinase. Glucose is phosphorylated to glucose-6-phosphate.

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11
Q

What is the name of the second irreversible reaction and third reaction overall. Explain it.

A

Phosphofructokinase. Fructose-6-phosphate is phosphorylated to generate fructose-1, 6-bisphosphate.

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12
Q

what reaction is an isoenzyme and allosteric?

A

Phosphofructokinase.

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13
Q

What does allosteric mean?

A

Enzymes that have an additional binding site for effector molecules other than the active site.

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14
Q

What is the name of the third irreversible reaction and final reaction overall? Explain it.

A

Pyruvate kinase. Phosphenolpyruvate to pyruvate.

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15
Q

What is substrate level phosphorylation?

A

It is when a phosphate group is transferred to ADP.

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16
Q

what happens to NADH in anaerobic conditions?

A

NADH is re-oxidised by lactate dehydrogenase as O2 is not available, which provides NAD+ for more glycolysis.

17
Q

what happens to NADH in aerobic conditions?

A

NADH is re-oxidised in the electron transport chain, making ATP in oxidative phosphorylation.