Glycolysis

Question 1

3 irreversible steps

Answer 1

Glu -> glu-6-p; glucokinase/hexokinase
Fruct-6-p ——-> fruct-1,6-bisp ; pfk
Pep ——-> pyruvate ; pyruvate kinase

Question 2

Glycolysis steps:

Answer 2

Glu —–> glu-6-p ——–> fruct-6-p ———> fruct-1,6-bisp –aldolase——> DHAP< —–>glycerald-3-p –dehydrogenase—-> 1,3-bisphosphoglycerate ——-> 3-pglycerate ——-> 2-p glycerate —-enolase—> pep ——–> pyruvate

Question 3

Floride bulb

Answer 3

Affects enolase inhibiting it and stopping glycolysis

Question 4

ATP net

Answer 4

4 formed
2 used
Net gain of 2 ATP & 2NADH

Question 5

Glucose only source of energy in

Answer 5

Brain

Matured RBC

Question 6

Glucokinase & hexokinase difference

Answer 6

Hexokinase:

• present in all cells
• have higher affinity
• have low Km
• act on all carbohydrates

Glucokinase:

• act only on glucose
• have low affinity
• high Km value
• present in liver
• act only when glucose is greater than 100mg/dl