glycolyse en gluconeogenese

Question 1

allosterische controle (spier) PHOSPHOFRUCTOKINASE

Answer 1

(-) ATP
(-) H+
(+) AMP

Question 2

allosterische controle (spier) HEXOKINASE

Answer 2

(-) GLU-6-P

Question 3

allosterische controle (spier) PYRUVAATKINASE

Answer 3

(-) ATP

(+) F-1,6-BP

Question 4

allosterische controle (spier) PYRUVAAT CARBOXYLASE

Answer 4

(-) ADP

Question 5

allosterische controle (spier) PHOSPOENOLPYRUVAAT CARBOXY KINASE

Answer 5

(-) ADP

Question 6

allosterische controle (spier) FRUCTOSE-1,6-BIPHOSPATHASE

Answer 6

(-) AMP

Question 7

glycolyse gluconeogenese SPIER

Answer 7

afhankelijk van energie charge ATP/AMP

-hoog = veel ATP = daling GLYCOLYSE & stijging GLUCONEOGENESE

Question 8

glycolyse gluconeogenese LEVER

Answer 8

• Aanwezigheid van bouwstenen biosynthese
• hoog = daling GLYCOLYSE & stijging GLUCONEOGENESE
• glucose concentratie in bloed
• hoog (insuline) = stijging GLYCOLYSE & daling GLUCONEOGENESE
• laag (glucagon) = daling GLYCOLYSE & stijging GLUCONEOGENESE

Question 9

allosterische controle (lever) PHOSPHOFRUCTOKINASE

Answer 9

(-) ATP
(-) citraat
(+)

Question 10

allosterische controle (lever) HEXOKINASE

Answer 10

(-) Glu-6-P

Question 11

allosterische controle (lever) GLUCOKINASE

Answer 11

heeft er geen

Question 12

allosterische controle (lever) PYRUVAATKINASE

Answer 12

(-) ATP
(-) Alanine
(+) F-1,6-BP

Question 13

allosterische controle (lever) PYRUVAAT CARBOXYLASE

Answer 13

(-) ADP

(+) acetyl-coA

Question 14

allosterische controle (lever) PHOSPOENOLPYRUVAAT CARBOXY KINASE

Answer 14

(-) ADP

Question 15

allosterische controle (lever) FRUCTOSE-1,6-BIPHOSPATHASE

Answer 15

(-) AMP
(-) F-2,6-BP
(+) citraat

Question 16

COVALENTE MODIFCIATIE LEVER

glucose in bloed = LAAG

Answer 16

1. Vrijstelling glucagon
2. activatie PKA
3. Fosforylatie PFK2–> inactivatie
4. Activatie FBPase2
5. [F-2,6-BP] daalt –> [F-6-P] stijgt -(isomerase)-> G-6-P -(G6pase)-> glucose
6. Glycolyse daalt & Gluconeogenese stijgt
   * fosforylatie pyruvaat kinase: phospoenolpyruvaat -x-> pyruvaat

Question 17

COVALENTE MODIFCIATIE LEVER

glucose in bloed = HOOG

Answer 17

1. Vrijstelling insuline
2. Activatie PP (protein phosphatase) via tyrosine kinase receptor
3. Defosforylatie PFK2–> activatie
4. Inactivatie FBPase2
5. [F-2,6-BP] stijgt–> [F-6-P] daalt -(isomerase)-> G-6-P -(G6pase)-> glucose
6. Glycolyse stijgt & Gluconeogenese daalt
   * defosforylatie pyruvaat kinase: phospoenolpyruvaat –> pyruvaat

Question 18

REGULATIE ENZYMHOEVEELHEID LEVER

glucose in bloed = LAAG –> Glucagon

Answer 18

1. Daling TRANSCRIPTIE
2. PFK1 daalt
3. PK daalt
4. PFK2/FBPase2 daalt
   => GLUCONEOGENSE stijgt & GLYCOLYSE daalt

Question 19

REGULATIE ENZYMHOEVEELHEID LEVER

glucose in bloed = HOOG –> Insuline

Answer 19

1. Stijging TRANSCRIPTIE
2. PFK1 stijgt
3. PK stijgt
4. PFK2/FBPase2 stijgt
   => GLUCONEOGENSE daalt & GLYCOLYSE stijgt

Question 20

Cori cyclus

Answer 20

Lactaat (spier) –> bloedbaan –> lever –> pyruvaat –> glucose –> bloedbaan –> spier –> pyruvaat –> lactaat