Glycogen Metabolism Flashcards
Main stores of glycogen?
Which stores larger amount of glycogen?
What does each of these stores use it for?
Muscle and liver
Muscle
Muscle=energy; liver= maintains blood glucose for liver and kidney
All straight chains have what kind of linkages?
1->4 glycosidic linkages
Difference between gluconeogenesis and glycogenesis
Gluconeogenesis= slow to react but reliable Glycogenesis= significant storage with a fast response but only lasts 12-14 hours
Two parts of your body that are dependent on glucose
Brain and RBC
Why can’t muscle glycogen provide blood glucose maintenance?
Due to absence of glucose-6-phosphatase
Where does glycogenesis take place?
What enzymes does glycogenesis require?
Cytosol
Requires glycogen synthase and branching enzyme
What is the rate limiting enzyme for synthesis of glycogen?
Glycogen synthase
What is needed to initiate glycogen synthesis?
Glycogen fragment/glycogenin - has tyrosine attached which auto-glycosylates (meaning it adds glucose molecules)
Give an example of glycosylation
HbA1C
In order to enter glycogenesis, why does glucose need to be activated to UDP glucose?
This activation is necessary so glucose does not enter glycolysis; enters glycogenesis instead (needed for branching enzyme and glycogen synthase to act)
So once you have UDP glucose- glycogen synthase comes and does what?
Elongation- can only act if you have glycogenin or other small chain of glucose. Also, only works on non-reducing ends
- What does it mean to be a nonreducing sugar?
2. Explain which sugars are reducing/nonreducing?
- Anomeric carbon is involved in formation of bond so it cannot oxidize/react (does not have an OH group)
- First sugar is reducing end; all other sugars are nonreducing (remember: nonreducing is important for synthesis of glycogen) Nonreducing ends are the very ends of branches in this case
When does branching enzyme come in?
What does it do?
When glucose chain is long enough
Forms new alpha 1->6 branch and breaks an old alpha 1->4 branch
Importance of what branching enzyme is doing?
Branching enzyme is making multiple branches. Branches are helpful because they are easier to digest and take up a smaller area (liver and muscle are unable to hold long, straight chains)
- Glycogen synthase makes what kind of bonds?
2. Branching enzyme makes what kind of bonds?
- Alpha (1->4) bonds (by further elongation at nonreducing ends)
- Alpha (1->6) bonds (by further branching)
~this eventually makes the branched structure that is glycogen