Glucose Metabolism

Question 0

GLUT2 provides basolateral glucose efflux for intestinal glucose absorption

Answer 0

Mice GLUT2 deficient compared to wild type
GLUT2 knockout mice had 55% higher glucose contents in intestinal tissue and 40% reduction in plasma glucose
Lack of GLUT2 impairs glucose efflux from intestine to blood

Question 1

ATP inhibits PFK

Answer 1

ATP concentration of 1mM reduced the activity of isolated hepatic PFK to 15% of that of 0.07mM ATP
Passonneau and Lowry

Question 2

GLUT4 receptors sequestered in vesicles

Answer 2

Slot et al
Immunohistochemistry
Under basal conditions 99% of GLUT4 labelling within the cell
Stimulation –> 40% of GLUT4 labelling randomly distributed on cell surface, exocytosis