Glucose

Question 1

Glucose oxidase reaction

Answer 1

A two-step reaction that results in the production of an intense brown colour measured at 425 to 475nm using a spectrophotometer. The absorbance is proportional to the original glucose concentration in patient serum.
o This reaction is susceptible to falsely decreased values with increased levels of uric acid, bilirubin, and ascorbic acid.

Question 2

hexokinase reaction

Answer 2

is more accurate than the glucose oxidase, glucose-6-phosphate dehydrogenase is highly specific and is not as susceptible to interference from uric acid or ascorbic acid, The rate of production is proportional to the amount of glucose present in the patient’s serum