Glial Cell Biology ILO 7 - Describe research techniques aimed to analyse the proteome and transcriptome of brain glial cells

Question 1

What are the main steps of isolation astrocytes from mouse brain?

Answer 1

1. Standardised tissue dissociation
2. Debris removal
3. Red blood cell removal
4. Magnetic isolation of astrocytes
5. Analysis by flow cytometry
6. Cell culture