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PMLS LEC 2 (2ND SEM) > GLASSWARES > Flashcards

GLASSWARES Flashcards

1
Q

corrodes metals, irritates skin and bleaches fabrics and clothing.

A

Hypochlorite

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2
Q

useful because its impact and corrosive resistance

A

Plastic

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3
Q

Has a disadvantage: some releases ions

A

Borosilicate glass

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4
Q

low heat and chemical resistance, inexpensive, use to make disposable Container

A

Flint glass

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5
Q

Describe borosilicate glass

A

low alkaline earth content, high thermal resistance (*600°C), ideal for use, free from chemical contaminants (i.e., heavy metals)

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6
Q

Kimax and pyrex

A

Borosilicate glass

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7
Q

It is Inexpensive and disposable

A

Polyethylene

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8
Q

Example of Polyethylene

A

Petri dish

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9
Q

It is sterilizable and expensive

A

Polypropylene

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10
Q

Ex of Polypropylene

A

Autoclavable syringe filters
Autoclavable plastics

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11
Q

Flask

A

Erlenmeyer flask
Florence flask
Volumetric flask

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12
Q

Pipet

A

Serological
Mohr
Volumetric

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13
Q

BASIC LABWARES

A

1 Beaker
2. Flask - Erlenmeyerflask, Florence flask, Volumetric flask
3. Pipet - Serological, Mohr, Volumetric
4. Graduated cylinder
5. Test tubes 6. Bottles
7. Others: Stirring rod, coplin jar, forceps, tongs, test tube holder, testtube rack, pipette bulb/pipettor/aspirator, syringes, vacutainer set

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14
Q

wide-mouth, straight sided container with a pouring spout formed from the rim.

A

Beaker

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15
Q

An upright, straight-sided container with a flared base that provides stability, octagon base.

A

Graduated cylinder

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16
Q

Markings of graduated cylinder

A

By increments

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17
Q

Uses of graduated cylinder

A

non-critical volume measurement
hold 24-hour urine specimen

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18
Q

GC: read at lower meniscus

A

Light colored

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19
Q

GC: read at upper meniscus

A

Dark colored solution

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20
Q

holds liquid samples
container for test reaction

A

Test tubes

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21
Q

vacutainer tubes top

A

Stoppered

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22
Q

screw-capped tubes top

A

Screw-on

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23
Q

Kinds of top test tubes

A

Open
Stoppered (close)
Screw-on (close)

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24
Q

Kinds of bottom test tubes

A

Round (general use)
Open (ESR)
Flat (culture)
Pointed (urinalysis

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25
Q

Routine chemical testing requiring
serum; blood bank and serological tests.

A

Red vacutainer tube

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26
Q

Chemically clean tube; general use

A

Amber vacutainer tube

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27
Q

Use for Tissue culture

A

Pink vacutainer tube

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28
Q

Use for hematology testing

A

Lavender/violet

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29
Q

ANTICOAGULANT ofyellow

A

Sodium Polyetholesulfonate (SPS)

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30
Q

ANTICOAGULANT of black VT

A

Oxalate

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31
Q

ANTICOAGULANT of lavender/violet VT

A

EDTA

32
Q

ANTICOAGULANT of light blue VT

A

Sodium citrate

33
Q

Coagulation testing

A

Light blue

34
Q

ANTICOAGULANT pf green VT

A

Heparin

35
Q

Chemistry testing

A

Green

36
Q

ANTICOAGULANT of royal blue VT

A

Sodium heparin/EDTA

37
Q

Use for chemistry trace elements

A

Royal blue

38
Q

flat bottom, sloping sides that gradually narrow its diameter so that
top opening is bottle-like, plain flat opening

A

Erlenmeyer flask

39
Q

flat bottom, round sides, long cylindrical neck o marking: total capacity

A

Florence flask

40
Q

very long neck, pear-shaped

A

Volumetric flask

41
Q

contain exact volume at specified temperature (for accurate measurement)

A

Volumetric flask

42
Q
  • curve fluid surface
A

Meniscus

43
Q

To measure and transfer liquid or solutions.

A

Pipettes

44
Q

Types pf pipettes

A

Measuring pipette
Transfer pipette
Pasteur pipette

45
Q

Used in old determination of hemoglobin

A

Sahli-hellige

46
Q

filled, allowed to drain and remaining tip fluid is blown-out
deliver exact amount; not rinsed-out

A

To blow-out

47
Q

Example of TBO/ To blow-out pipette

A

Oswald-folin
-measure viscous fluid like whole blood
-special pipette

Serological pipette
-long glass tube with uniform diameter
- markings extend to the delivery tip
- has two frosted band near the mouth piece

48
Q

-refilled or rinse-out after draining by appropriate solvent
-hold exact amount of liquid that must be completely transferred by rinsing for accurate measurement.

A

To contain(TC) / Rinse-out pipette

49
Q
  • allow liquid to drain by gravity
    • pipette is held vertically and the tip is place against the side of accepting vessel
A

To deliver (TD)

50
Q

Measuring pipettes are divided into

A

Mohr pipette
Serological pipette

51
Q

the graduations on these always end before the tip

A

Mohr pipette

52
Q

the graduation marks continue to the tip

A

Serological pipette

53
Q

Advantages of Using Automatic Pipette:

A

Easy to operate
Reduce technical error

54
Q

o most common type with disposable tip, piston-operated device
o fixed oradiustable volume

A

Eppendorf micropipette

55
Q

• delivers cylindrical slender glass rods, for mixing manually solutions o heat-resistant

A

SMI Micropipettor

56
Q

used with volumetric and standard graduated pipettes (serological of various sizes.

A

Electric/battery-operated pipette

57
Q

can deliver different volumes

A

Battery-operated/electric pipette

58
Q

functional from storing to mixing reagents

A

Test tubes

59
Q

one of the most important apparatus

A

Test tubes

60
Q

rpm means

A

Revolutions per minute

61
Q

a small centrifuge used in blood banking and serology to spin serological tubes

A

Serofuge

62
Q

spins special microtubes at high speed

A

Microfuge

63
Q

spins capillary tubes at high speeds so that hematocrit can be measured.

A

Microhematocrit centrifuge

64
Q

used to separate chylomicrons fromserum,fractionate lipoproteins, perform drug binding assays, preparation of tissue for steroid hormone receptor assay.

A

Ultracentrifuge

65
Q

high speed &sampleskeep cool while being centrifuge (i.e. ultracentrifuge), use inresearch but not required for routine use.

A

Refrigerated centrifuge

66
Q

container for sterilize items.

A

Autoclave chamber

67
Q

surrounds the chamber and source ofsteam

A

Metal jacket

68
Q

securely lock & has a seal to prevent escape ofsteam.

A

Door or cover

69
Q

separate for temperature and pressure.

A

Gauges

70
Q

for weighing chemicals and media

A

Laboratory balance

71
Q

Never open the door unless chamber pressure is:

A

Zero psi

72
Q

asingle beam with equal arms, standard weights are added manually to the right side to counter balance the object weight.

A

Double pan (DIRECT COMPARISON)

73
Q

arms are unequal in length, object is placed on the short arm pan and arestoring force mechanicallyapplied tothe other armuntil indicator is balanced.

A

Single Pan (SUBSTITUTION)

74
Q

Important Parts of the Autoclave:

A

Autoclave chamber
Metal jacket
Door / cover
Gauges

75
Q

TEMPERATURE CONTROLLED CHAMBERS

A

1) Above ambient or room temperature
a. oven
b. water bath (heating bath)
c. incubator

2) Cold temperature
a. refrigerator
b. freezer

76
Q

Principle of digital weighing scale

A

Electromagnetic force

PMLS LEC 2 (2ND SEM) (4 decks)

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