GIT infections Flashcards
How can GI pathogens cause damage?
Local inflammation
Ulceration/perforation of mucosal epithelium
Disruption of normal microbiota
Pharmacological action of bacterial toxins
Invasion to blood or lymphatics
What bacteria can cause diarrhoea and what are their incubation periods, durations and symptoms?
Variable incubation period
Campylobacter - 2-11 days
Shigella - 1-4 days
Variable duration
Campylobacter - up to 3w
Shigella - 2-3 days
Variable symptoms
Campylobacter and Shigella - bloody stools
EPEC and cholera - watery stools
Vibrio cholerae
Gram -ve, characterised by epidemics and pandemics, flourishes where no clean drinking water/sewage disposal
Based on O-antigens (O1 associated with early pandemics, non-o1 associated with recent outbreaks
Vaccines - parenteral has low protective efficiency, oral vaccine suitable for travellers
Only infective in large doses, many organisms killed in stomach, colonisation of SI involving flagellar motion, mucinase, attachment to specific receptors, loss of fluid and electrolytes without damage to enterocytes
Consequences of cholera infection
Fluid loss of up to 1l/hr
Electrolyte imbalance leading to dehydration, metabolic acidosis and hypokalemia
Hypovolaemic shock
40-60% mortality but <1% if ORT given
Escherichia Coli
Gram -ve, normal GI microbiota, some strains cause virulence factors enabling them to cause disease
Examples are
EPEC (enteropathogenic) - sporadic cases and outbreaks of infection in <5y/o
ETEC (enterotoxigenic) - traveller’s diarrhoea
VTEC/STEC - sporadic cases and outbreaks of gastroenteritis
EIEC (enteroinvasive) - food-borne infection in areas of poor hygiene (often persistent diarrhoea)
What are the adherence strategies of E.coli and what are the mode of actions?
Pili/fimbriae
Pedestal formation
Mode of actions are:
LT - heat labile toxin
STa - heat stable toxin
Campylobacter jejuni
Gram -ve, causes food-associated diarrhoea, large animal reservoir, transmission through consumption of raw meat and contaminated milk, mucosal inflammation and fluid secretion
Inflammation involves entire mucosa, villous atrophy, necrotic debris in crypts, thickening of basement membrane
Salmonella spp
Gram -ve, food-associated diarrhoea through raw/uncooked meat, secondary spread can be human-human
Important species are S. typhi, S. paratyphi and S, enteritidis
Absorption to epithelial cells in terminal SI, penetration of cells and migration to lamina propria, infamm response mediates response of prostaglandins, CAMP stimulation, release of fluid and electrolytes causing diarrhoea
S. typhi and S. paratyphi causes enteric fevers, systemic infections initiated in GIT, multiply within and transported around body in macrophages
Typhoid fever and typhoid vaccines
Patient can excrete S. typhi in faeces for several weeks after recovery, 1-3% become chronic carriers so it is a notifiable disease
vaccines are either oral live attenuated (booster after 5y) or parenteral (booster after 2y), recommended for travellers to endemic areas
Shigella spp
Causes shigellosis (bacillary dysentery), human only pathogen 4 species - S. dysenteriae, S. flexneri, S.boydii, S. sonnei
Attaches to mucosal epithelium of distal ileum and colon, inflammation and ulceration, rarely invasive, diarrhoea watery initially but later can contain blood and mucus, usually self-limiting
Listeria monocytogenes
Food-borne pathogen associated with pate, soft cheese etc, pregnant women/elderly/immunosuppressed at risk, usually presents as meningitis
Antibiotic associated diarrhoea
Disruption of gut microbiota
Tetracycline - allows colonisation by S.aureus and candida sp
CLindamycin allows C.difficile to multiply
C.difficile infection now associated with vanc resistance
C.difficile infection
Produces spores for survival
Produces an enterotoxin and cytotoxin
Clostridium perfringens
Food-associated GI infection, spores survive cooking and germination takes place
Multiplication in LI, production of spores and enterotoxin, damage to int epithelium, diarrhoea
Rotavirus
Commonest in children <2, commonest in winter, transmission faeco-oral but may also be faeco-respiratory
Intubation period 1-2 days, replication of virus in SI epithelal cells at tips of villi, damage caused to infected cells leaving immature cells with reduced absorptive capacity for sugar, water and electrolytes
Onset of vomiting lasting 4-7 days
Oral vaccine