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421 - Micro Lab > GI tract > Flashcards

GI tract Flashcards

1
Q

CVA media

A

Cefoperozone, vancomycin, amphitericin B

48 hr incubation for Campylobacter

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2
Q

Yersinia enterocolitica

A

Growth on MAC at RT
Motility at RT
CIN media: bulleye

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3
Q

Virus cause GI infection

A 
Adenovirus
Astrovirus
Norovirus
Rotavirus
Sapovirus
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4
Q

O antigen

A

LPS layer

Heat stabile

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5
Q

H antigen

A

Flagellar antigen

Heat labile

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6
Q

Vi antigen

A

Capsular polysaccharide

Mask O antigen if present –> boiling 15’ to destroy Vi and expose O antigen

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7
Q

Salmonella type

A

Salmonella bongori: cold-blooded animal, environment
Salmonella enterica:
- 6 subspecies
- human pathogen: S. enterica ssp enterica (serovars: typhi, paratyphi, enteriditis, typhimurium)

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8
Q

Salmonella on different media

A

SS Chrome: back center with clear perimeter
HE: black colonies
MAC: translucent - NLF
SMAC: pink - SF

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9
Q

Salmonella biochem test

A

TSI: K/A, G, Pos
LIA: P/P
Urea: Neg

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10
Q

Shigella on different media

A

SS Chrome: teal green
HE: translucent
MAC: translucent
SMAC: translucent

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11
Q

Shigella biochem test

A

TSI: K/A, NG, Neg
LIA; P/Y
Urea: Neg
Motility: Neg

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12
Q

Campylobacter condition & morphology

A

Microaerophilic, Capnophilic
Growth at 42C
Flat, irregular, moist, spreading edge
GS: GNR, “gull-wing” shaped

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13
Q

Campylobacter biochemistry test

A

Oxidase: Pos
Motility: darting
Hippurate test: C. jejuni: Pos vs C. coli: Neg
Selective media: CVA

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14
Q

4 antisera for Shigella

A 
A = S. dysenteriae
B = S. flexneri
C = S. boydii
D = S. sonnei
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15
Q

S. sonnei compared with others

A

Ornithine: Pos
ONPG: Pos

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16
Q

CIN media

A

Cefsulodin, Irgasan, Novobiocin

17
Q

Plesidomonas

A

GNR
Oxidase: Pos
Glucose fermenter

18
Q

Aeromonas

A

GNR
Oxidase: Pos
Glucose fermenter

19
Q

Vibrio

A 
GNR, comma shapped
R/O summer time
TCBS media
Oxidase pos
Glucose fermenter
20
Q

Lactose fermenter Enterobacteriaceae on SS Chrome

A

CEEK + Yersinia spp.

Pink colonies

21
Q

Distinguish E. coli 0157: H7

A

SMAC : no color –> Non sorbitol fermenter

421 - Micro Lab (6 decks)

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