Germination

Question 1

What does the barley endosperm represent in the context of brewing?

Answer 1

A fortress protecting starch granules inside

Question 2

What is the role of Carboxypeptidase and β-Glucan Solubilase on Day 1?

Answer 2

Weaken the walls by breaking down the protein layer

Question 3

Which enzyme sneaks inside the barley endosperm but needs help to fully break down starch?

Answer 3

β-Amylase

Question 4

What happens on Day 2 during the barley breakdown process?

Answer 4

Endo Peptidase & β-Glucanases tear apart the protein matrix around the starch granules

Question 5

What enzyme attacks small starch granules from Days 2 to 4?

Answer 5

α-Amylase

Question 6

What is the function of Limit Dextrinase during Days 4-6?

Answer 6

Finishes the job, breaking down the branched starch (amylopectin)

Question 7

Fill in the blank: On Day 1, the walls weaken through the action of _______ and _______.

Answer 7

[Carboxypeptidase], [β-Glucan Solubilase]

Question 8

True or False: The starch is fully exposed and converted into sugars by the end of Day 6.

Answer 8

True

Question 9

List the sequence of enzyme actions from Day 1 to Day 6.

Answer 9

• Day 1 – Walls weaken (Carboxypeptidase, β-Glucan Solubilase, β-Amylase)
• Day 2 – Shields fall (Endo Peptidase, β-Glucanases)
• Days 2-4 – Starch is unlocked (α-Amylase)
• Days 4-6 – Final cleanup (Limit Dextrinase)

Question 10

What is the final result of the enzyme actions on barley?

Answer 10

The starch is fully exposed and converted into sugars, fueling the embryo’s growth and preparing the malt for brewing