Gerber Microarrays Flashcards
1
Q
what does Northern and Southern blot detect
A
presence of a SINGLE gene
2
Q
Northen blot is for
A
RNA
3
Q
Southern blot is for
A
DNA
4
Q
describe Northern/Southern blotting
A
- digest w/ restriction enzymes
- separate fragments on a gel
- transfer to membrane
- DNA= immobilised
- radioactively labelled probe used to detect target gene
- can see size and expression level
5
Q
what is hybridisation
A
complementary nucleic acids seq between 2 DNA or a DNA and RNA strand form H bonds between
6
Q
what is a DNA microarray
A
slides that contain thousands of tiny spots at defined positions, with each spot containing a known DNA sequence or gene
7
Q
what is the purpose of microarrays
A
so you can detect and quantify allgenes in SAMPLE by HYBRIDISATION at the same time: DNA (GENE/GENE VARIANT PRESENCE) or RNA (GENE ACTIVITY/ TRANSCRIPTOME )
