Genomics, Transcriptomics, and Proteomics

Question 1

Define genome, transcriptome, and proteome and discuss how these are studied.

Answer 1

.

Question 2

Discuss the contextual relationship between the genome, transcriptome, and proteome as it relates to when and where (time and which cells) are used as a study subject.

Answer 2

.

Question 3

Describe the Sanger/Dideoxy DNA sequencing reaction?

Answer 3

.

Question 4

Describe the “shot gun” approach to sequencing an entire genome.

Answer 4

.

Question 5

Define a SNP?

Answer 5

.

Question 6

Describe a DNA microarray and identify the different type of microarrays?

Answer 6

.

Question 7

Discuss how DNA microarrays can be used to identify SNPs, differences in gene expression between two cell types, and how they can be used to determine the location of expressed genes on a chromosome.

Answer 7

.

Question 8

Define “western blot”.

Answer 8

.

Question 9

Describe the process of western blotting. Indicate how a specific protein is identified and what is used as a probe.

Answer 9

.

Question 10

Describe the process of 2D protein electrophoresis? Define isoelectric point.

Answer 10

.

Question 11

Discuss some of the findings of the various genome projects.

Answer 11

.

Question 12

Define RFLP, and SNP.

Answer 12

.

Question 13

Define FISH.

Answer 13

.

Question 14

Describe how FISH can be used as a diagnostic tool to identify chromosomal abnormalities and to determine the orderly arrangement of genes.

Answer 14

.

Question 15

Describe how transcriptional changes are detected by:

• PCR-based methods (RT-PCR, cDNA-AFLP)
• Hybridization based (macro and microarray, two channel and tiling)
• Sequence based (SAGE and MPSS)

Answer 15

.