Genome Projects

Question 1

genome

Answer 1

the complete set of genetic information contained in the cells of an organism

Question 2

genome sequencing

Answer 2

means working out the complete DNA base sequence for the entire DNA of an organism
-allows us to determine the amino acid sequence for the poly peptides coded for by that DNA.

Question 3

proteome

Answer 3

the complete set of proteins that can be produced by a cell

Question 4

sequencing of proteomes in simple organisms

Answer 4

-simple organisms, like prokaryotes do not contain introns in their DNA
-means that the genome can be used directly to sequence the proteins that derive from the proteome of an organism

Question 5

use of proteome sequencing in simple organisms

Answer 5

identifying potential antigens for use in vaccine production

Question 6

sequencing of proteomes in complex organisms

Answer 6

-complex organisms contain non-coding DNA->introns and regulatory genes, it is much harder to obtain the proteome

Question 7

applications of genome sequencing

Answer 7

-comparing genomes between species to determine evolutionary relationships.
-genetic matching
-personalised medicine
-synthetic biology

Question 8

genome sequencing

Answer 8

used to be a manual process
-methods used to sequence genomes are continuously being improved and updated-has now become fully automated
-a reaction mixture is created and after the process is complete, a machine reads the base sequence.